DC Field | Value | Language |
---|---|---|
dc.contributor.author | DANIEL, M | ko |
dc.contributor.author | CHOI, JH | ko |
dc.contributor.author | Kim, Jung-Hoe | ko |
dc.contributor.author | LEBEAULT, JM | ko |
dc.date.accessioned | 2013-02-27T06:53:48Z | - |
dc.date.available | 2013-02-27T06:53:48Z | - |
dc.date.created | 2012-02-06 | - |
dc.date.created | 2012-02-06 | - |
dc.date.issued | 1992-09 | - |
dc.identifier.citation | APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, v.37, no.6, pp.702 - 706 | - |
dc.identifier.issn | 0175-7598 | - |
dc.identifier.uri | http://hdl.handle.net/10203/67096 | - |
dc.description.abstract | Pseudomonas 135, a facultative methylotroph, was cultivated on methanol as a sole carbon and energy source for the accumulation of poly-beta-hydroxybutyric acid (PHB). The cells grew fairly well on minimal synthetic medium containing 0.5% (v/v) of methanol at pH 7.0 and 30-degrees-C. The maximum specific growth rate was determined to be 0.26-0.28 h-1 with a growth yield of 0.38 in the optimized growth medium. For stimulation of PHB accumulation in the cells, deficiency of nutrients such as NH4+, Mg2+ and PO43- was crucial even though cell growth was significantly suppressed. The PHB content of a 40-h culture was determined to be 37% of the total cell mass in NH4+-limited medium, 42.5% on Mg2+-deficient medium, and 34.5% on PO43- -deficient medium. The maximum content of PHB in the cells could reach 55% in NH4+-limited fed-batch culture. The average relative molecular eight determined by gel permeation chromatography was 3.7 x 1O(5) in NH4+-limited culture, 2.5 x 10(5) in Mg2+-deficient mediUm, and 3.1 x 10(5) in PO43--deficient medium. Polydispersity determined in each culture was relatively high (about 10-(11)). The solid PHB had a melting temperatue of 173-degrees-C. | - |
dc.language | English | - |
dc.publisher | SPRINGER | - |
dc.title | EFFECT OF NUTRIENT DEFICIENCY ON ACCUMULATION AND RELATIVE MOLECULAR-WEIGHT OF POLY-BETA-HYDROXYBUTYRIC ACID BY METHYLOTROPHIC BACTERIUM, PSEUDOMONAS-135 | - |
dc.type | Article | - |
dc.identifier.wosid | A1992JR13200003 | - |
dc.identifier.scopusid | 2-s2.0-0026775673 | - |
dc.type.rims | ART | - |
dc.citation.volume | 37 | - |
dc.citation.issue | 6 | - |
dc.citation.beginningpage | 702 | - |
dc.citation.endingpage | 706 | - |
dc.citation.publicationname | APPLIED MICROBIOLOGY AND BIOTECHNOLOGY | - |
dc.contributor.localauthor | Kim, Jung-Hoe | - |
dc.contributor.nonIdAuthor | DANIEL, M | - |
dc.contributor.nonIdAuthor | CHOI, JH | - |
dc.contributor.nonIdAuthor | LEBEAULT, JM | - |
dc.type.journalArticle | Article | - |
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