DC Field | Value | Language |
---|---|---|
dc.contributor.author | Szybalski, W | ko |
dc.contributor.author | Kim, Sun-Chang | ko |
dc.contributor.author | Hasan, N | ko |
dc.contributor.author | Podhajska, AJ | ko |
dc.date.accessioned | 2013-02-25T22:19:07Z | - |
dc.date.available | 2013-02-25T22:19:07Z | - |
dc.date.created | 2012-02-06 | - |
dc.date.created | 2012-02-06 | - |
dc.date.issued | 1991-04 | - |
dc.identifier.citation | GENE, v.100, pp.13 - 26 | - |
dc.identifier.issn | 0378-1119 | - |
dc.identifier.uri | http://hdl.handle.net/10203/65702 | - |
dc.description.abstract | Class-IIS restriction enzymes (ENases-IIS) interact with two discrete sites on double-stranded DNA: the recognition site, which is 4-7 bp long, and the cleavage site, usually 1-20 bp away from the recognition site. The recognition sequences of ENases-IIS are totally (or partially) asymmetric and all of the characterized ENases-IIS are monomeric. A total of 35 ENases-IIS are described (80, if all isoschizomers are taken into consideration) together with ten related ENases (class IIT), and 15 cognate methyltransferases (MTases-IIS). The physical, chemical, and molecular properties of the ENases-IIS and MTases-IIS are reviewed and many unique applications of this class of enzymes are described, including: precise trimming of DNA; retrieval of cloned fragments; gene assembly; use as a universal restriction enzyme; cleavage of single-stranded DNA; detection of point mutations; tandem amplification; printing-amplification reaction; and localization of methylated bases. | - |
dc.language | English | - |
dc.publisher | ELSEVIER SCIENCE BV | - |
dc.subject | SITE-SPECIFIC ENDONUCLEASE | - |
dc.subject | DNA MODIFICATION METHYLTRANSFERASES | - |
dc.subject | LOCATING METHYLATED BASES | - |
dc.subject | DOUBLE-HELICAL DNA | - |
dc.subject | RECOGNITION SEQUENCE | - |
dc.subject | BACILLUS-BREVIS | - |
dc.subject | NUCLEOTIDE-SEQUENCE | - |
dc.subject | MODIFICATION SYSTEM | - |
dc.subject | FLAVOBACTERIUM-OKEANOKOITES | - |
dc.subject | HAEMOPHILUS-GALLINARUM | - |
dc.title | Class-IIS restriction enzymes--a review | - |
dc.type | Article | - |
dc.identifier.wosid | A1991FV37300003 | - |
dc.identifier.scopusid | 2-s2.0-0025797325 | - |
dc.type.rims | ART | - |
dc.citation.volume | 100 | - |
dc.citation.beginningpage | 13 | - |
dc.citation.endingpage | 26 | - |
dc.citation.publicationname | GENE | - |
dc.identifier.doi | 10.1016/0378-1119(91)90345-C | - |
dc.contributor.localauthor | Kim, Sun-Chang | - |
dc.contributor.nonIdAuthor | Szybalski, W | - |
dc.contributor.nonIdAuthor | Hasan, N | - |
dc.contributor.nonIdAuthor | Podhajska, AJ | - |
dc.type.journalArticle | Review | - |
dc.subject.keywordAuthor | RECOMBINANT DNA | - |
dc.subject.keywordAuthor | ENDONUCLEASES | - |
dc.subject.keywordAuthor | METHYLTRANSFERASES | - |
dc.subject.keywordAuthor | PROBES | - |
dc.subject.keywordAuthor | RECOGNITION AND CLEAVAGE DOMAINS | - |
dc.subject.keywordAuthor | TRIMMING VECTORS | - |
dc.subject.keywordPlus | SITE-SPECIFIC ENDONUCLEASE | - |
dc.subject.keywordPlus | DNA MODIFICATION METHYLTRANSFERASES | - |
dc.subject.keywordPlus | LOCATING METHYLATED BASES | - |
dc.subject.keywordPlus | DOUBLE-HELICAL DNA | - |
dc.subject.keywordPlus | RECOGNITION SEQUENCE | - |
dc.subject.keywordPlus | BACILLUS-BREVIS | - |
dc.subject.keywordPlus | NUCLEOTIDE-SEQUENCE | - |
dc.subject.keywordPlus | MODIFICATION SYSTEM | - |
dc.subject.keywordPlus | FLAVOBACTERIUM-OKEANOKOITES | - |
dc.subject.keywordPlus | HAEMOPHILUS-GALLINARUM | - |
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