Stability of the Lipase Immobilized on DEAE-Sephadex for Continuous Lipid Hydrolysis in Organic Solvent

Abstract

Continuous hydrolysis reaction was carried out in glass-column by using lipase from Candida rugosa immobilized on DEAE-Sephadex A50. Substrate olive oil dissolved in hydrophobic organic solvent (isooctane) was supplied into the reactor with a concurrent stream of aqueous buffer. Stability of the immobilized enzyme was greatly increased in higher substrate concentration. Glycerol added in the aqueous stream showed stabilizing effect against the organic solvent; half life was extended from 220 hrs to 450 hrs by 15% glycerol supplemented at 30-degrees-C.