Initial culture conditions affect the sensitivity of HepG2 cells to excessive mechanical agitation

Abstract

Hepatoma cells, HepG2, grew normally on microcarriers even at a relatively high agitation rate if sufficient time was allowed for cell attachment and adhesion. However, if a high agitation rate was applied shortly after initial cell attachment, the growth rate was retarded. This sensitivity to mechanical agitation appears to be dependent on the inoculation cell density. © 1989 Kluwer Academic Publishers.