To efficiently supply inhibitory aromatic solvents such as benzene, toluene, and xylenes in liquid phase for aerobic biodegradation processes, a novel technique was developed. Silicon tubing was immersed in the bioreactor and liquid solvent was circulated within the tubing from a solvent reservoir. Transfer rate of the solvent through a silicon tubing was significantly affected by impeller speed, whereas other operating parameters such as aeration rate and circulation rate of liquid solvent within the tubing showed a negligible effect. Aerobic biodegradation of toluene and p-xylene was carried out using this method and the biodegradation rate of solvent mixture was high compared to those of conventional methods.