DC Field | Value | Language |
---|---|---|
dc.contributor.author | Helfman, David M | ko |
dc.contributor.author | Roscigno, Robert F. | ko |
dc.contributor.author | Mulligan, George J. | ko |
dc.contributor.author | Finn, Linda A. | ko |
dc.contributor.author | Weber, athy S. | ko |
dc.date.accessioned | 2013-02-25T01:09:57Z | - |
dc.date.available | 2013-02-25T01:09:57Z | - |
dc.date.created | 2012-02-06 | - |
dc.date.created | 2012-02-06 | - |
dc.date.issued | 1990-04 | - |
dc.identifier.citation | GENES AND DEVELOPMENT, v.4, no.1, pp.98 - 110 | - |
dc.identifier.issn | 0890-9369 | - |
dc.identifier.uri | http://hdl.handle.net/10203/58651 | - |
dc.description.abstract | The rat β-tropomyosin gene encodes two isoforms, termed skeletal muscle β-tropomyosin and fibroblast plast tropomyosin 1 (TM-1), via an alternative RNA processing mechanism. The gene contains 11 exons. Exons 1-5 and exons 8 and 9 are common to all mRNAs expressed from the gene. Exons 6 and 11 are used in fibroblasts, as well as smooth muscle , whereas exons 7 and 10 are used only in skeletal muscle. In the present studies we focused on the mutually exclusive internal alternative splice choice involving exon 6 (fibroblast-type splice) and exon 7 (skeletal muscle-type splice). We have indentified two distinct elements in the intron, upstream of exon 7, involved in splice site selection. The first element is comprised of a polypyrimidine tract located 89-143 nucleotides upstream of the 3 splice site, which specifies the location of the lariat branchpoints used, 144-153 nucleotides upstream of exon 7. The 3 splice site AG dinucleotide has no role in selection of these branchpoints. The second element is comprised of intron sequences located between the polypyrimidine tract and the 3 splice site of exon 7. It contains an important determinant in alternative splice site selection, because deletion of these sequences results in the use of the skeletal muscle-specific exon in nonmuscle cells. We propose that the use of lariat branchpoints located far upstream from 3 splice site may be a general feature of some alternatively excised introns, reflecting the presence of regulatory sequences located between the lariat branch site and the 3 splice site. The data also indicate that alternative splicing of the rat β-tropomyosin gene is regulated by a somewhat different mechanism from that described for rat α-tropomyosin gene and the transformer-2 gene of Drosophila melanogaster | - |
dc.language | English | - |
dc.publisher | COLD SPRING HARBOR LABORATORY PRESS | - |
dc.title | Identification of two distinct intron elements involved in alternative splicing of beta-tropomyosin pre-mRNA | - |
dc.type | Article | - |
dc.identifier.scopusid | 2-s2.0-0025125897 | - |
dc.type.rims | ART | - |
dc.citation.volume | 4 | - |
dc.citation.issue | 1 | - |
dc.citation.beginningpage | 98 | - |
dc.citation.endingpage | 110 | - |
dc.citation.publicationname | GENES AND DEVELOPMENT | - |
dc.contributor.localauthor | Helfman, David M | - |
dc.contributor.nonIdAuthor | Roscigno, Robert F. | - |
dc.contributor.nonIdAuthor | Mulligan, George J. | - |
dc.contributor.nonIdAuthor | Finn, Linda A. | - |
dc.contributor.nonIdAuthor | Weber, athy S. | - |
dc.subject.keywordAuthor | Alternative splicing | - |
dc.subject.keywordAuthor | RNA processing | - |
dc.subject.keywordAuthor | tropomyosin | - |
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