EFFECT OF TRISODIUM CITRATE TREATMENT ON HYBRIDOMA CELL VIABILITY

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Trisodium citrate has been widely used to dissolve calcium alginate beads in order to estimate cell concentration in the beads. To obtain an accurate measurement of viable cell concentration in calcium alginate beads, the effect of trisodium citrate solutions on hybridoma cell viability was studied with regard to stage of growth and trisodium citrate concentration. The cells in the decline phase of growth were more sensitive to 30 minutes of trisodium citrate treatment than the cells in the exponential phase of growth. The cell viability did not decrease rapidly during citrate treatment regardless of cell growth phase and trisodium citrate concentration in the range of 1-1.5%. By using the commercially available sodium alginate, Keltone LV, dissolution time can be kept short enough (below 5 minutes) to keep the effect of trisodium citrate negligible to cell viability. © 1991 Science & Technology Letters.
Publisher
Kluwer Academic Publishers
Issue Date
1991-07
Language
English
Article Type
Article
Keywords

ANIMAL-CELLS; IMMOBILIZATION; ANTIBODY; ALGINATE

Citation

BIOTECHNOLOGY TECHNIQUES, v.5, no.4, pp.295 - 298

ISSN
0951-208X
DOI
10.1007/BF02438666
URI
http://hdl.handle.net/10203/58120
Appears in Collection
BS-Journal Papers(저널논문)
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