Effect of temperature on plasmid stability and expression of cloned cellulase gene in a recombinant Bacillusmegaterium

Abstract

The expression of carboxymethyl cellulase gene in Bacillusmegaterium (pCK108) was investigated with respect to temperature in batch culture. The suboptimal temperature supporting maximal cell growth rate was determined to be 30 °C at which stability of the plasmid pCK108 could be maintained stable. However, cellular plasmid contents, production rate of cellulase of the cell, and efficiency of the gene expression increased significantly with increase of the temperature from 30 °C to 44 °C, even though the plasmid stability decreased up to 60% level at the end of the culture. © 1987 Science and Technology Letters.