Metal ions differentially influence the aggregation and deposition of Alzheimer's beta-amyloid on a solid template

Cited 203 time in webofscience Cited 0 time in scopus
  • Hit : 664
  • Download : 14
DC FieldValueLanguage
dc.contributor.authorHa, Chankiko
dc.contributor.authorRyu, Jungkiko
dc.contributor.authorPark, Chan Beumko
dc.date.accessioned2008-07-15T01:35:49Z-
dc.date.available2008-07-15T01:35:49Z-
dc.date.created2012-02-06-
dc.date.created2012-02-06-
dc.date.issued2007-05-
dc.identifier.citationBIOCHEMISTRY, v.46, no.20, pp.6118 - 6125-
dc.identifier.issn0006-2960-
dc.identifier.urihttp://hdl.handle.net/10203/5803-
dc.description.abstractThe abnormal deposition and aggregation of beta-amyloid (A beta) on brain tissues are considered to be one of the characteristic neuropathological features of Alzheimer's disease (AD). Environmental conditions such as metal ions, pH, and cell membranes are associated with A beta deposition and plaque formation. According to the amyloid cascade hypothesis of AD, the deposition of A beta 42 oligomers as diffuse plaques in vivo is an important earliest event, leading to the formation of fibrillar amyloid plaques by the further accumulation of soluble A beta under certain environmental conditions. In order to characterize the effect of metal ions on amyloid deposition and plaque growth on a solid surface, we prepared a synthetic template by immobilizing A beta oligomers onto a N-hydroxysuccinimide ester-activated solid surface. According to our study using ex situ atomic force microscopy (AFM), Fourier transform infrared spectroscopy (FT-IR), and thioflavin T (ThT) fluorescence spectroscopy, Cu2+ and Zn2+ ions accelerated both A beta 40 and A beta 42 deposition but resulted only in the formation of "amorphous" aggregates. In contrast, Fe3+ induced the deposition of "fibrillar" amyloid plaques at neutral pH. Under mildly acidic environments, the formation of fibrillar amyloid plaques was not induced by any metal ion tested in this work. Using secondary ion mass spectroscopy (SIMS) analysis, we found that binding Cu ions to A beta deposits on a solid template occurred by the possible reduction of Cu ions during the interaction of A beta with Cu2+. Our results may provide insights into the role of metal ions on the formation of fibrillar or amorphous amyloid plaques in AD.-
dc.description.sponsorshipThis study was supported by grants from the National Institutes of Health (1R21AG024114-01A2) and the Korea Research Foundation (KRF-2006-D00078).en
dc.languageEnglish-
dc.language.isoen_USen
dc.publisherAmer Chemical Soc-
dc.subjectATOMIC-FORCE MICROSCOPY-
dc.subjectA-BETA-
dc.subjectFIBRIL FORMATION-
dc.subjectSHEET FORMATION-
dc.subjectDISEASE-
dc.subjectPEPTIDE-
dc.subjectCOPPER-
dc.subjectBRAIN-
dc.subjectZINC-
dc.subjectIRON-
dc.titleMetal ions differentially influence the aggregation and deposition of Alzheimer's beta-amyloid on a solid template-
dc.typeArticle-
dc.identifier.wosid000246456200025-
dc.identifier.scopusid2-s2.0-34249041646-
dc.type.rimsART-
dc.citation.volume46-
dc.citation.issue20-
dc.citation.beginningpage6118-
dc.citation.endingpage6125-
dc.citation.publicationnameBIOCHEMISTRY-
dc.embargo.liftdate9999-12-31-
dc.embargo.terms9999-12-31-
dc.contributor.localauthorPark, Chan Beum-
dc.contributor.nonIdAuthorHa, Chanki-
dc.contributor.nonIdAuthorRyu, Jungki-
dc.type.journalArticleArticle-
dc.subject.keywordPlusATOMIC-FORCE MICROSCOPY-
dc.subject.keywordPlusA-BETA-
dc.subject.keywordPlusFIBRIL FORMATION-
dc.subject.keywordPlusSHEET FORMATION-
dc.subject.keywordPlusDISEASE-
dc.subject.keywordPlusPEPTIDE-
dc.subject.keywordPlusCOPPER-
dc.subject.keywordPlusBRAIN-
dc.subject.keywordPlusZINC-
dc.subject.keywordPlusIRON-
Appears in Collection
MS-Journal Papers(저널논문)
Files in This Item
This item is cited by other documents in WoS
⊙ Detail Information in WoSⓡ Click to see webofscience_button
⊙ Cited 203 items in WoS Click to see citing articles in records_button

qr_code

  • mendeley

    citeulike


rss_1.0 rss_2.0 atom_1.0