DC Field | Value | Language |
---|---|---|
dc.contributor.author | Ha, Chanki | ko |
dc.contributor.author | Ryu, Jungki | ko |
dc.contributor.author | Park, Chan Beum | ko |
dc.date.accessioned | 2008-07-15T01:35:49Z | - |
dc.date.available | 2008-07-15T01:35:49Z | - |
dc.date.created | 2012-02-06 | - |
dc.date.created | 2012-02-06 | - |
dc.date.issued | 2007-05 | - |
dc.identifier.citation | BIOCHEMISTRY, v.46, no.20, pp.6118 - 6125 | - |
dc.identifier.issn | 0006-2960 | - |
dc.identifier.uri | http://hdl.handle.net/10203/5803 | - |
dc.description.abstract | The abnormal deposition and aggregation of beta-amyloid (A beta) on brain tissues are considered to be one of the characteristic neuropathological features of Alzheimer's disease (AD). Environmental conditions such as metal ions, pH, and cell membranes are associated with A beta deposition and plaque formation. According to the amyloid cascade hypothesis of AD, the deposition of A beta 42 oligomers as diffuse plaques in vivo is an important earliest event, leading to the formation of fibrillar amyloid plaques by the further accumulation of soluble A beta under certain environmental conditions. In order to characterize the effect of metal ions on amyloid deposition and plaque growth on a solid surface, we prepared a synthetic template by immobilizing A beta oligomers onto a N-hydroxysuccinimide ester-activated solid surface. According to our study using ex situ atomic force microscopy (AFM), Fourier transform infrared spectroscopy (FT-IR), and thioflavin T (ThT) fluorescence spectroscopy, Cu2+ and Zn2+ ions accelerated both A beta 40 and A beta 42 deposition but resulted only in the formation of "amorphous" aggregates. In contrast, Fe3+ induced the deposition of "fibrillar" amyloid plaques at neutral pH. Under mildly acidic environments, the formation of fibrillar amyloid plaques was not induced by any metal ion tested in this work. Using secondary ion mass spectroscopy (SIMS) analysis, we found that binding Cu ions to A beta deposits on a solid template occurred by the possible reduction of Cu ions during the interaction of A beta with Cu2+. Our results may provide insights into the role of metal ions on the formation of fibrillar or amorphous amyloid plaques in AD. | - |
dc.description.sponsorship | This study was supported by grants from the National Institutes of Health (1R21AG024114-01A2) and the Korea Research Foundation (KRF-2006-D00078). | en |
dc.language | English | - |
dc.language.iso | en_US | en |
dc.publisher | Amer Chemical Soc | - |
dc.subject | ATOMIC-FORCE MICROSCOPY | - |
dc.subject | A-BETA | - |
dc.subject | FIBRIL FORMATION | - |
dc.subject | SHEET FORMATION | - |
dc.subject | DISEASE | - |
dc.subject | PEPTIDE | - |
dc.subject | COPPER | - |
dc.subject | BRAIN | - |
dc.subject | ZINC | - |
dc.subject | IRON | - |
dc.title | Metal ions differentially influence the aggregation and deposition of Alzheimer's beta-amyloid on a solid template | - |
dc.type | Article | - |
dc.identifier.wosid | 000246456200025 | - |
dc.identifier.scopusid | 2-s2.0-34249041646 | - |
dc.type.rims | ART | - |
dc.citation.volume | 46 | - |
dc.citation.issue | 20 | - |
dc.citation.beginningpage | 6118 | - |
dc.citation.endingpage | 6125 | - |
dc.citation.publicationname | BIOCHEMISTRY | - |
dc.embargo.liftdate | 9999-12-31 | - |
dc.embargo.terms | 9999-12-31 | - |
dc.contributor.localauthor | Park, Chan Beum | - |
dc.contributor.nonIdAuthor | Ha, Chanki | - |
dc.contributor.nonIdAuthor | Ryu, Jungki | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordPlus | ATOMIC-FORCE MICROSCOPY | - |
dc.subject.keywordPlus | A-BETA | - |
dc.subject.keywordPlus | FIBRIL FORMATION | - |
dc.subject.keywordPlus | SHEET FORMATION | - |
dc.subject.keywordPlus | DISEASE | - |
dc.subject.keywordPlus | PEPTIDE | - |
dc.subject.keywordPlus | COPPER | - |
dc.subject.keywordPlus | BRAIN | - |
dc.subject.keywordPlus | ZINC | - |
dc.subject.keywordPlus | IRON | - |
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