DC Field | Value | Language |
---|---|---|
dc.contributor.author | Chae, Jung-Il | ko |
dc.contributor.author | Cho, Young Keun | ko |
dc.contributor.author | Cho, Seong-Keun | ko |
dc.contributor.author | Kim, Jin-Hoi | ko |
dc.contributor.author | Han, Yong Mahn | ko |
dc.contributor.author | Koo, Deog-Bon | ko |
dc.contributor.author | Lee, Kyung-Kwang | ko |
dc.date.accessioned | 2008-04-08T02:27:45Z | - |
dc.date.available | 2008-04-08T02:27:45Z | - |
dc.date.created | 2012-02-06 | - |
dc.date.created | 2012-02-06 | - |
dc.date.issued | 2008-02 | - |
dc.identifier.citation | BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, v.366, no.2, pp.379 - 387 | - |
dc.identifier.issn | 0006-291X | - |
dc.identifier.uri | http://hdl.handle.net/10203/3717 | - |
dc.description.abstract | The potential medical applications of animal cloning include xenotransplantation, but the complex molecular cascades that control porcine organ development are not fully understood. Still, it has become apparent that organs derived from cloned pigs may be suitable for transplantation into humans. In this study, we examined the pancreas of an adult cloned pig developed through somatic cell nuclear transfer (SCNT) using two-dimensional electrophoresis (2-DE) and Western blotting. Proteomic analysis revealed 69 differentially regulated proteins, including such apoptosis-related species as annexins, lamins, and heat shock proteins, which were unanimously upregulated in the SCNT sample. Among the downregulated proteins in SCNT pancreas were peroxiredoxins and catalase. Western blot results indicate that several antioxidant enzymes and the anti-apoptotic protein were downregulated in SCNT pancreas, whereas several caspases were upregulated. Together, these data suggest that the accumulation of reactive oxygen species (ROS) in the pancreas of an adult cloned pig leads to apoptosis. (C) 2007 Elsevier Inc. All rights reserved. | - |
dc.description.sponsorship | This study was supported by Grants from the BioGreen 21 Program (20070401034017) and the KBRDG Initiative Research Program (F104AD010004-06A0401-00410) of the Rural Development Administration and Ministry of Science and Technology, Republic of Korea. | en |
dc.language | English | - |
dc.language.iso | en_US | en |
dc.publisher | ACADEMIC PRESS INC ELSEVIER SCIENCE | - |
dc.subject | HEAT-SHOCK PROTEINS | - |
dc.subject | OXIDATIVE STRESS | - |
dc.subject | INDUCED APOPTOSIS | - |
dc.subject | UP-REGULATION | - |
dc.subject | C-MYC | - |
dc.subject | CLONING | - |
dc.subject | GROWTH | - |
dc.subject | DEATH | - |
dc.subject | BCL-2 | - |
dc.subject | ACTIVATION | - |
dc.title | Proteomic analysis of pancreas derived from adult cloned pig | - |
dc.type | Article | - |
dc.identifier.wosid | 000252436300018 | - |
dc.identifier.scopusid | 2-s2.0-37449024072 | - |
dc.type.rims | ART | - |
dc.citation.volume | 366 | - |
dc.citation.issue | 2 | - |
dc.citation.beginningpage | 379 | - |
dc.citation.endingpage | 387 | - |
dc.citation.publicationname | BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | - |
dc.identifier.doi | 10.1016/j.bbrc.2007.11.114 | - |
dc.embargo.liftdate | 9999-12-31 | - |
dc.embargo.terms | 9999-12-31 | - |
dc.contributor.localauthor | Han, Yong Mahn | - |
dc.contributor.nonIdAuthor | Chae, Jung-Il | - |
dc.contributor.nonIdAuthor | Cho, Young Keun | - |
dc.contributor.nonIdAuthor | Cho, Seong-Keun | - |
dc.contributor.nonIdAuthor | Kim, Jin-Hoi | - |
dc.contributor.nonIdAuthor | Koo, Deog-Bon | - |
dc.contributor.nonIdAuthor | Lee, Kyung-Kwang | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordAuthor | proteomic analysis | - |
dc.subject.keywordAuthor | somatic cell cloning | - |
dc.subject.keywordAuthor | pancreas | - |
dc.subject.keywordAuthor | ROS | - |
dc.subject.keywordAuthor | apoptosis | - |
dc.subject.keywordAuthor | pig | - |
dc.subject.keywordPlus | HEAT-SHOCK PROTEINS | - |
dc.subject.keywordPlus | OXIDATIVE STRESS | - |
dc.subject.keywordPlus | INDUCED APOPTOSIS | - |
dc.subject.keywordPlus | UP-REGULATION | - |
dc.subject.keywordPlus | C-MYC | - |
dc.subject.keywordPlus | CLONING | - |
dc.subject.keywordPlus | GROWTH | - |
dc.subject.keywordPlus | DEATH | - |
dc.subject.keywordPlus | BCL-2 | - |
dc.subject.keywordPlus | ACTIVATION | - |
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