Change of plasmid-specified RNA metabolism in a temperature-sensitive escherichia coli rnp a mutant strain온도 감수성 rnpA 돌연변이 대장균에서 플라스미드 RNA 대사의 변화

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Escherichia coli RNase P is a processing enzyme which cleaves tRNA precursors at the 5``-end of the tRNA sequence. It is composed of protein component, C5 protein, and RNA component, M1 RNA. M1 RNA synthesized from a ColEl-related plasmid, which harbors the rnpB gene encoding M1 RNA, was accumulated in E. coli strain A49 carrying the thermosensitive mutation in the rnp A gene encoding C5 protein at nonpermissive temperature. This accumulation of M1 RNA accompanied the accumulation of two small RNAs in the A49 cells. They were designated as RNA X and RNA Y in diminishing size. Their nucleotide sequences were determined by the enzymatic method. Analysis of the RNA sequences showed that RNA X and RNA Y are composed of 96 and 67 nucleotides, respectively and related with RNA I, which is the negative controller of ColE1 plasmid replication. Surprisingly RNA X has the same sequences of RNA Y but differs in containing an extra 29 nucleotides at the 5`` end. These results indicate that RNA X and RNA Y are originated from the ColE1-related plasmid, and suggest that a defect in RNase P function by the rnpA49 mutation is responsible for accumulation of these RNAs, implying the possible involvement of RNase P in controlling the replication of ColE1-type plasmids.
Advisors
Lee, Young-Hoonresearcher이영훈researcher
Description
한국과학기술원 : 화학과,
Publisher
한국과학기술원
Issue Date
1992
Identifier
59918/325007 / 000901493
Language
eng
Description

학위논문(석사) - 한국과학기술원 : 화학과, 1992.2, [ iv, 41 p. ]

URI
http://hdl.handle.net/10203/32175
Link
http://library.kaist.ac.kr/search/detail/view.do?bibCtrlNo=59918&flag=dissertation
Appears in Collection
CH-Theses_Master(석사논문)
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