DC Field | Value | Language |
---|---|---|
dc.contributor.advisor | 임성갑 | - |
dc.contributor.author | Lee, Minseok | - |
dc.contributor.author | 이민석 | - |
dc.date.accessioned | 2024-07-25T19:30:17Z | - |
dc.date.available | 2024-07-25T19:30:17Z | - |
dc.date.issued | 2022 | - |
dc.identifier.uri | http://library.kaist.ac.kr/search/detail/view.do?bibCtrlNo=1044804&flag=dissertation | en_US |
dc.identifier.uri | http://hdl.handle.net/10203/320404 | - |
dc.description | 학위논문(박사) - 한국과학기술원 : 생명화학공학과, 2022.2,[viii, 100 p. :] | - |
dc.description.abstract | In vitro cell culture, a method capable of studying the behavior of animal cells in a controlled environment, can be the basis for various studies, including biomedical engineering and regenerative medicine. To date, various in vitro cell culture methods such as 3D cell culture have been developed in order to more closely mimic the in vivo system, but these still have some limitations including lack of in vivo relevance and low differentiation efficiency according to cell types. In this thesis, we control the interaction between the cells and the culture substrate based on a polymer thin film by using an initiated chemical vapor deposition (iCVD), and thereby construct a functional culture model of various cell types. By precisely controlling the adhesion ability of cells through the hydrophobic polymer, we developed a bone marrow-derived dendritic cell culture platform capable of promoting differentiation and growth. In addition, by controlling the surface properties of the culture substrate and corresponding properties of the adsorbed protein, we developed a cancer spheroid culture method that can mimic the interaction between cells and proteins existing in the tumor microenvironment, and applied it to construct a microfluidic-based metastasis model. | - |
dc.language | eng | - |
dc.publisher | 한국과학기술원 | - |
dc.subject | 체외 세포 배양▼a개시제를 이용한 화학 기상 증착법▼a수지상 세포▼a암 스페로이드▼a랩온어칩 | - |
dc.subject | In vitro cell culture▼aInitiated chemical vapor deposition▼aDendritic cell▼aCancer spheroid▼aLab-on-a-chip | - |
dc.title | Establishment of functional cell culture model by controlling interactions between cells and polymer thin film based culture substrate | - |
dc.title.alternative | 고분자 박막 기반의 배양 기판과 세포 간의 상호 작용 조절을 통한 기능적 세포 배양 모델 확립 | - |
dc.type | Thesis(Ph.D) | - |
dc.identifier.CNRN | 325007 | - |
dc.description.department | 한국과학기술원 :생명화학공학과, | - |
dc.contributor.alternativeauthor | Im, Sung Gap | - |
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