DC Field | Value | Language |
---|---|---|
dc.contributor.author | Koh, Hyun Gi | ko |
dc.contributor.author | Chang, Yong Keun | ko |
dc.contributor.author | Kang, Nam Kyu | ko |
dc.date.accessioned | 2024-07-01T09:00:12Z | - |
dc.date.available | 2024-07-01T09:00:12Z | - |
dc.date.created | 2024-06-25 | - |
dc.date.issued | 2024-02 | - |
dc.identifier.citation | JOURNAL OF APPLIED PHYCOLOGY, v.36, no.1, pp.233 - 241 | - |
dc.identifier.issn | 0921-8971 | - |
dc.identifier.uri | http://hdl.handle.net/10203/320090 | - |
dc.description.abstract | Microalgae have emerged as a promising feedstock for the sustainable production of lipid-based products, including biofuels and omega-3 fatty acids, by utilizing CO2 as a carbon source. Glycerol-3-phosphate dehydrogenase (GPDH) is a vital enzyme in microalgal lipid synthesis, converting dihydroxyacetone phosphate into glycerol-3-phosphate, the initial substrate for the Kennedy pathway responsible for triacylglycerol production. In this study, we identified and overexpressed a chloroplastic NAD(P)(+)-dependent GPDH (NsGPDH1) in Nannochloropsis salina. Quantitative reverse transcription polymerase chain reaction and western blotting revealed increased NsGPDH1 expression in the transformants as compared to wild-type N. salina. This overexpression of NsGPDH1 resulted in increased glycerol production and a higher proportion of mono-unsaturated fatty acids, which are indicative of GPDH overexpression. Notably, lipid productivity in NsGPDH1-overexpressing N. salina strains increased by 40% without significant growth defects. These results highlight the effectiveness of NsGPDH1 overexpression as a strategy for enhancing lipid biosynthesis in N. salina, contributing to the advancement of microalgae-based lipid production. | - |
dc.language | English | - |
dc.publisher | SPRINGER | - |
dc.title | Enhancing lipid productivity in Nannochloropsis salina by overexpression of endogenous glycerol-3-phosphate dehydrogenase | - |
dc.type | Article | - |
dc.identifier.wosid | 001156584100003 | - |
dc.identifier.scopusid | 2-s2.0-85177457117 | - |
dc.type.rims | ART | - |
dc.citation.volume | 36 | - |
dc.citation.issue | 1 | - |
dc.citation.beginningpage | 233 | - |
dc.citation.endingpage | 241 | - |
dc.citation.publicationname | JOURNAL OF APPLIED PHYCOLOGY | - |
dc.identifier.doi | 10.1007/s10811-023-03141-6 | - |
dc.contributor.localauthor | Chang, Yong Keun | - |
dc.contributor.nonIdAuthor | Koh, Hyun Gi | - |
dc.contributor.nonIdAuthor | Kang, Nam Kyu | - |
dc.description.isOpenAccess | N | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordAuthor | Lipid | - |
dc.subject.keywordAuthor | Biofuel | - |
dc.subject.keywordAuthor | Glycerol-3-phosphate-dehydrogenase | - |
dc.subject.keywordAuthor | Glycerol | - |
dc.subject.keywordAuthor | Nannochloropsis | - |
dc.subject.keywordAuthor | Eustigmatophyceae | - |
dc.subject.keywordPlus | GREEN MICROALGA | - |
dc.subject.keywordPlus | TRIACYLGLYCEROL BIOSYNTHESIS | - |
dc.subject.keywordPlus | DIHYDROXYACETONE PHOSPHATE | - |
dc.subject.keywordPlus | CHLAMYDOMONAS | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordPlus | CLONING | - |
dc.subject.keywordPlus | ALGAE | - |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.