DC Field | Value | Language |
---|---|---|
dc.contributor.author | Kim Hansol | ko |
dc.contributor.author | Lee Seoyoung | ko |
dc.contributor.author | Ju Yong | ko |
dc.contributor.author | Kim Hyoyong | ko |
dc.contributor.author | Jang Hyowon | ko |
dc.contributor.author | Park Yeonkyung | ko |
dc.contributor.author | Lee Sang Mo | ko |
dc.contributor.author | Yong Dongeun | ko |
dc.contributor.author | Kang Taejoon | ko |
dc.contributor.author | Park Hyun Gyu | ko |
dc.date.accessioned | 2024-06-25T12:00:08Z | - |
dc.date.available | 2024-06-25T12:00:08Z | - |
dc.date.created | 2024-06-25 | - |
dc.date.issued | 2024-06 | - |
dc.identifier.citation | BIOSENSORS & BIOELECTRONICS, v.253 | - |
dc.identifier.issn | 0956-5663 | - |
dc.identifier.uri | http://hdl.handle.net/10203/320011 | - |
dc.description.abstract | We herein present a multifunctional self-priming hairpin probe-based isothermal amplification, termed MSH, enabling one-pot detection of target nucleic acids. The sophisticatedly designed multifunctional self-priming hairpin (MSH) probe recognizes the target and rearranges to prime itself, triggering the amplification reaction powered by the continuously repeated extension, nicking, and target recycling. As a consequence, a large number of double-stranded DNA (dsDNA) amplicons are produced that could be monitored in real-time using a dsDNAintercalating dye. Based on this unique design approach, the nucleocapsid (N) and the open reading frame 1 ab (ORF1ab) genes of SARS-CoV-2 were successfully detected down to 1.664 fM and 0.770 fM, respectively. The practical applicability of our method was validated by accurately diagnosing 60 clinical samples with 93.33% sensitivity and 96.67% specificity. This isothermal one-pot MSH technique holds great promise as a point-of-care testing protocol for the reliable detection of a wide spectrum of pathogens, particularly in resource-limited settings. | - |
dc.language | English | - |
dc.publisher | ELSEVIER ADVANCED TECHNOLOGY | - |
dc.title | Multifunctional self-priming hairpin probe-based isothermal nucleic acid amplification and its applications for COVID-19 diagnosis | - |
dc.type | Article | - |
dc.identifier.wosid | 001205793700001 | - |
dc.type.rims | ART | - |
dc.citation.volume | 253 | - |
dc.citation.publicationname | BIOSENSORS & BIOELECTRONICS | - |
dc.contributor.localauthor | Park Hyun Gyu | - |
dc.contributor.nonIdAuthor | Kim Hansol | - |
dc.contributor.nonIdAuthor | Lee Seoyoung | - |
dc.contributor.nonIdAuthor | Ju Yong | - |
dc.contributor.nonIdAuthor | Kim Hyoyong | - |
dc.contributor.nonIdAuthor | Jang Hyowon | - |
dc.contributor.nonIdAuthor | Park Yeonkyung | - |
dc.contributor.nonIdAuthor | Lee Sang Mo | - |
dc.contributor.nonIdAuthor | Yong Dongeun | - |
dc.contributor.nonIdAuthor | Kang Taejoon | - |
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