DC Field | Value | Language |
---|---|---|
dc.contributor.author | Yoon, Junhyeok | ko |
dc.contributor.author | Lee, Jinhwan | ko |
dc.contributor.author | Kim, Jaemin | ko |
dc.contributor.author | Lee, Sang Mo | ko |
dc.contributor.author | Kim, Soohyun | ko |
dc.contributor.author | Park, Hyun Gyu | ko |
dc.date.accessioned | 2024-06-25T12:00:07Z | - |
dc.date.available | 2024-06-25T12:00:07Z | - |
dc.date.created | 2024-06-25 | - |
dc.date.created | 2024-06-25 | - |
dc.date.issued | 2024-06 | - |
dc.identifier.citation | BIOSENSORS & BIOELECTRONICS, v.253 | - |
dc.identifier.issn | 0956-5663 | - |
dc.identifier.uri | http://hdl.handle.net/10203/320009 | - |
dc.description.abstract | We herein present a novel ultrasensitive RNase H assay based on phosphorothioated-terminal hairpin formation and self-priming extension (PS-THSP) reaction. The detection probe employed as a key component in this technique serves as a substrate for RNase H and triggers the PS-THSP reaction upon the RNase H-mediated degradation of the probe. As a consequence, a large number of long concatemeric amplification products could be produced and used to identify the RNase H activity through the fluorescence signals produced by the nucleic acid-specific fluorescent dye, SYTO 9. Importantly, the use of the gp32 protein allowed the PS-THSP reaction to be performed at 37 degrees C, ultimately enabling an isothermal one-step RNase H assay. Based on this sophisticated design principle, the RNase H activity was very sensitively detected, down to 0.000237 U mL-1 with high specificity. We further verified its practical applicability through its successful application to the screening of RNase H inhibitors. With its operational convenience and excellent analytical performance, this technique could serve as a new platform for RNase H assay in a wide range of biological applications. | - |
dc.language | English | - |
dc.publisher | ELSEVIER ADVANCED TECHNOLOGY | - |
dc.title | A novel ultrasensitive RNase H assay based on phosphorothioated-terminal hairpin formation and self-priming extension reaction | - |
dc.type | Article | - |
dc.identifier.wosid | 001218643700001 | - |
dc.identifier.scopusid | 2-s2.0-85186509471 | - |
dc.type.rims | ART | - |
dc.citation.volume | 253 | - |
dc.citation.publicationname | BIOSENSORS & BIOELECTRONICS | - |
dc.identifier.doi | 10.1016/j.bios.2024.116174 | - |
dc.contributor.localauthor | Park, Hyun Gyu | - |
dc.contributor.nonIdAuthor | Kim, Jaemin | - |
dc.contributor.nonIdAuthor | Kim, Soohyun | - |
dc.description.isOpenAccess | N | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordAuthor | RNase H | - |
dc.subject.keywordAuthor | Enzyme assay | - |
dc.subject.keywordAuthor | Phosphorothioate (PS) DNA | - |
dc.subject.keywordAuthor | Isothermal amplification | - |
dc.subject.keywordPlus | RIBONUCLEASE H | - |
dc.subject.keywordPlus | CATALYTIC MECHANISM | - |
dc.subject.keywordPlus | KINETIC-ANALYSIS | - |
dc.subject.keywordPlus | INHIBITORS | - |
dc.subject.keywordPlus | DNA | - |
dc.subject.keywordPlus | ENZYME | - |
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