DC Field | Value | Language |
---|---|---|
dc.contributor.author | Choi, Yo-han | ko |
dc.contributor.author | Kim, Woo-joong | ko |
dc.contributor.author | Lee, Dongwoo | ko |
dc.contributor.author | Jung, Bum-joon | ko |
dc.contributor.author | Shin, Eui-Cheol | ko |
dc.contributor.author | Lee, Wonhee | ko |
dc.date.accessioned | 2024-06-11T09:00:50Z | - |
dc.date.available | 2024-06-11T09:00:50Z | - |
dc.date.created | 2024-06-11 | - |
dc.date.created | 2024-06-11 | - |
dc.date.created | 2024-06-11 | - |
dc.date.issued | 2024-03 | - |
dc.identifier.citation | BioChip Journal | - |
dc.identifier.issn | 1976-0280 | - |
dc.identifier.uri | http://hdl.handle.net/10203/319739 | - |
dc.description.abstract | Among human CD8(+) T cells, senescent cells are marked by the expression of CD57. The frequency of senescent CD57(+)CD8(+) T cells is significantly correlated with aging and age-associated disorders, and it can be measured by multi-color flow cytometry. However, multi-color flow cytometry presents challenges in terms of accessibility and requires significant resource allocation. Therefore, developing a rapid and straightforward method for quantifying CD57(+)CD8(+) T cells remains a key challenge. This study introduces a microfluidic device composed of a PDMS microfluidic channel with a pre-modified glass substrate for anti-CD8 antibody immobilization. This design allows blood samples to flow through, enabling the selective capture of CD8(+) T cells while minimizing the required blood sample volume. This technology enables accurate and reliable quantification of CD57(+) cells among captured CD8(+) T cells through fluorescence image analysis. The ability of the device to easily quantify senescent CD57(+)CD8(+) T cells is anticipated to contribute significantly to both immunological research and clinical applications. | - |
dc.language | English | - |
dc.publisher | Springer | - |
dc.title | Microfluidic Capture Device for Simple, Cell Surface Marker-Based Quantification of Senescent CD8+ T Cells | - |
dc.type | Article | - |
dc.identifier.wosid | 001178156200003 | - |
dc.identifier.scopusid | 2-s2.0-85186891375 | - |
dc.type.rims | ART | - |
dc.citation.publicationname | BioChip Journal | - |
dc.identifier.doi | 10.1007/s13206-024-00142-6 | - |
dc.contributor.localauthor | Shin, Eui-Cheol | - |
dc.contributor.localauthor | Lee, Wonhee | - |
dc.contributor.nonIdAuthor | Lee, Dongwoo | - |
dc.contributor.nonIdAuthor | Jung, Bum-joon | - |
dc.description.isOpenAccess | N | - |
dc.type.journalArticle | Article; Early Access | - |
dc.subject.keywordAuthor | Affinity-based cell capture | - |
dc.subject.keywordAuthor | Cell surface marker | - |
dc.subject.keywordAuthor | Immunosenescence | - |
dc.subject.keywordAuthor | Microfluidic device | - |
dc.subject.keywordAuthor | Fluorescence imaging | - |
dc.subject.keywordPlus | CIRCULATING TUMOR-CELLS | - |
dc.subject.keywordPlus | FLOW | - |
dc.subject.keywordPlus | MICROCHIP | - |
dc.subject.keywordPlus | CD28(-) | - |
dc.subject.keywordPlus | CHIP | - |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.