DC Field | Value | Language |
---|---|---|
dc.contributor.author | Kim, Eun-Kyung | ko |
dc.contributor.author | Kwon, Kang-Beom | ko |
dc.contributor.author | Shin, Byung-Cheul | ko |
dc.contributor.author | Seo, Eun-A | ko |
dc.contributor.author | Lee, Young-Rae | ko |
dc.contributor.author | Kim, Jong-Suk | ko |
dc.contributor.author | Park, Jin-Woo | ko |
dc.contributor.author | Park, Byung-Hyun | ko |
dc.contributor.author | Ryu, Do-Gon | ko |
dc.date.accessioned | 2024-03-26T09:00:14Z | - |
dc.date.available | 2024-03-26T09:00:14Z | - |
dc.date.created | 2024-03-26 | - |
dc.date.created | 2024-03-26 | - |
dc.date.issued | 2005-07 | - |
dc.identifier.citation | LIFE SCIENCES, v.77, no.7, pp.824 - 836 | - |
dc.identifier.issn | 0024-3205 | - |
dc.identifier.uri | http://hdl.handle.net/10203/318875 | - |
dc.description.abstract | Scopoletin (6-methoxy-7-hydroxycoumarin) is a phenolic coumarin and a member of the phytoalexins. In this study we investigated whether scopoletin caused apoptosis in HL-60 promyelocytic cells and, if so, by what mechanisms. We found that scopoletin induced apoptosis as confirmed by a characteristic ladder pattern of discontinuous DNA fragments in a dose-dependent manner. The signal cascade activated by scopoletin included the heterodimeric redox-sensitive transcription factor NF-kappa B, which exhibited an upregulation of nuclear factor-kappa B (NF-kappa B) translocation to the nucleus by increase of I kappa B alpha degradation. In addition, scopoletin activated caspase-3 as was evidenced by both the proteolytic cleavage of the proenzyme and increased protease activity. Activation of caspase-3 resulted in the cleavage of 116 kDa poly(ADP-ribose) polymerase (PARP) to 85 kDa cleavage product in time-and dose-dependent fashions. Prior treatment of the cells with pyrrolidine dithiocarbamate, a potent inhibitor of NF-kappa B activation, or Ac-DEVD-CHO, a specific caspase-3 inhibitor, prevented scopoletin-induced caspase-3 activation, PARP cleavage, and finally DNA fragmentation. Taken together, these results suggest that scopoletin induces NF-kappa B activation, which, in turn, causes activation of caspase-3, degradation of PARP, and eventually leads to apoptotic cell death in HL-60 cells. | - |
dc.language | English | - |
dc.publisher | PERGAMON-ELSEVIER SCIENCE LTD | - |
dc.title | Scopoletin induces apoptosis in human promyeloleukemic cells, accompanied by activations of nuclear factor κB and caspase-3 | - |
dc.type | Article | - |
dc.identifier.wosid | 000229885100009 | - |
dc.identifier.scopusid | 2-s2.0-20344390242 | - |
dc.type.rims | ART | - |
dc.citation.volume | 77 | - |
dc.citation.issue | 7 | - |
dc.citation.beginningpage | 824 | - |
dc.citation.endingpage | 836 | - |
dc.citation.publicationname | LIFE SCIENCES | - |
dc.identifier.doi | 10.1016/j.lfs.2005.02.003 | - |
dc.contributor.localauthor | Park, Byung-Hyun | - |
dc.contributor.nonIdAuthor | Kim, Eun-Kyung | - |
dc.contributor.nonIdAuthor | Kwon, Kang-Beom | - |
dc.contributor.nonIdAuthor | Shin, Byung-Cheul | - |
dc.contributor.nonIdAuthor | Seo, Eun-A | - |
dc.contributor.nonIdAuthor | Lee, Young-Rae | - |
dc.contributor.nonIdAuthor | Kim, Jong-Suk | - |
dc.contributor.nonIdAuthor | Park, Jin-Woo | - |
dc.contributor.nonIdAuthor | Ryu, Do-Gon | - |
dc.description.isOpenAccess | N | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordAuthor | scopoletin | - |
dc.subject.keywordAuthor | HL-60 cells | - |
dc.subject.keywordAuthor | apoptosis | - |
dc.subject.keywordAuthor | NF-kappa B | - |
dc.subject.keywordAuthor | caspase-3 | - |
dc.subject.keywordPlus | TRANSCRIPTION FACTOR | - |
dc.subject.keywordPlus | HYDROGEN-PEROXIDE | - |
dc.subject.keywordPlus | INHIBITION | - |
dc.subject.keywordPlus | SUBUNIT | - |
dc.subject.keywordPlus | DNA | - |
dc.subject.keywordPlus | PROLIFERATION | - |
dc.subject.keywordPlus | CONSTITUENT | - |
dc.subject.keywordPlus | INVOLVEMENT | - |
dc.subject.keywordPlus | PROTEOLYSIS | - |
dc.subject.keywordPlus | MECHANISMS | - |
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