DC Field | Value | Language |
---|---|---|
dc.contributor.author | Kim, Mi-Ran | ko |
dc.contributor.author | Zhou, Lu | ko |
dc.contributor.author | Park, Byung-Hyun | ko |
dc.contributor.author | Kim, Jung Ryul | ko |
dc.date.accessioned | 2024-03-22T07:03:14Z | - |
dc.date.available | 2024-03-22T07:03:14Z | - |
dc.date.created | 2024-03-21 | - |
dc.date.issued | 2011-09 | - |
dc.identifier.citation | MOLECULAR MEDICINE REPORTS, v.4, no.5, pp.929 - 934 | - |
dc.identifier.issn | 1791-2997 | - |
dc.identifier.uri | http://hdl.handle.net/10203/318809 | - |
dc.description.abstract | Sulforaphane is one of the most abundant isothiocyanates found in certain cruciferous vegetables, particularly broccoli. To date, sulforaphane has gained attention as a chemopreventive compound. The mechanism responsible for the anticancer effects of sulforaphane in osteosarcoma, however, is not clear. In this study, we demonstrate an anti-proliferative mechanism of sulforaphane in human osteosarcoma cells. The treatment of cells with sulforaphane resulted in a concentration- and time-dependent inhibition of growth and G(2)/M phase arrest of the cell cycle. This effect was associated with a decrease in protein expression of cyclin A and B1 and their activating partners, cyclin-dependent kinases (CDKs) 1 and 2, with concomitant up-regulation of p21, a CDK inhibitor. Sulforaphane treatment also resulted in apoptosis as evidenced by an increase in annexin V+/propidium (V+/PI-) cells, the cleavage of 116-k Da poly (ADP-ribose) polymerase (PARP) and ICAD and oligonucleosomal DNA fragmentation. Taken together, these findings indicate that the molecular mechanisms underlying sulforaphane-mediated growth inhibition in U2-OS cells may be the modulation of the cell cycle machinery and the induction of apoptosis. | - |
dc.language | English | - |
dc.publisher | SPANDIDOS PUBL LTD | - |
dc.title | Induction of G2/M arrest and apoptosis by sulforaphane in human osteosarcoma U2-OS cells | - |
dc.type | Article | - |
dc.identifier.wosid | 000293435300024 | - |
dc.identifier.scopusid | 2-s2.0-79960400916 | - |
dc.type.rims | ART | - |
dc.citation.volume | 4 | - |
dc.citation.issue | 5 | - |
dc.citation.beginningpage | 929 | - |
dc.citation.endingpage | 934 | - |
dc.citation.publicationname | MOLECULAR MEDICINE REPORTS | - |
dc.identifier.doi | 10.3892/mmr.2011.520 | - |
dc.contributor.localauthor | Park, Byung-Hyun | - |
dc.contributor.nonIdAuthor | Kim, Mi-Ran | - |
dc.contributor.nonIdAuthor | Zhou, Lu | - |
dc.contributor.nonIdAuthor | Kim, Jung Ryul | - |
dc.description.isOpenAccess | N | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordAuthor | sulforaphane | - |
dc.subject.keywordAuthor | cell cycle | - |
dc.subject.keywordAuthor | apoptosis | - |
dc.subject.keywordAuthor | U2-05 | - |
dc.subject.keywordAuthor | osteosarcoma | - |
dc.subject.keywordPlus | CYCLE CONTROL | - |
dc.subject.keywordPlus | INHIBITORS | - |
dc.subject.keywordPlus | CHEMOTHERAPY | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordPlus | PROTECTS | - |
dc.subject.keywordPlus | DEATH | - |
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