DC Field | Value | Language |
---|---|---|
dc.contributor.author | Kim, Eun-Kyung | ko |
dc.contributor.author | Kwon, Kang-Beom | ko |
dc.contributor.author | Han, Mi-Jeong | ko |
dc.contributor.author | Song, Mi-Young | ko |
dc.contributor.author | Lee, Ji-Hyun | ko |
dc.contributor.author | Ko, Youn-Suk | ko |
dc.contributor.author | Shin, Byung-Cheal | ko |
dc.contributor.author | Yu, Jiahua | ko |
dc.contributor.author | Lee, Young-Rae | ko |
dc.contributor.author | Ryu, Do-Gon | ko |
dc.contributor.author | Park, Jin-Woo | ko |
dc.contributor.author | Park, Byung-Hyun | ko |
dc.date.accessioned | 2024-03-22T05:01:37Z | - |
dc.date.available | 2024-03-22T05:01:37Z | - |
dc.date.created | 2024-03-21 | - |
dc.date.created | 2024-03-21 | - |
dc.date.issued | 2007-07 | - |
dc.identifier.citation | INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE, v.20, no.1, pp.123 - 128 | - |
dc.identifier.issn | 1107-3756 | - |
dc.identifier.uri | http://hdl.handle.net/10203/318703 | - |
dc.description.abstract | Scutellaria barbata has been used to treat cancer in Chinese medicine. The responsible anticancer mechanism, however, is not clear. Here we demonstrated an inhibitory mechanism due to a Scutellaria barbata extract (SBE) on a human promyelocytic leukemia cell line (HL-60) that has a mutation in the tumor suppressor gene p53. HL-60 cells were incubated with various concentrations of SBE. After a 24-h incubation, cytotoxicity and apoptosis were determined by MTT and DNA fragmentation assay, respectively. After treatment with SBE, cell cycle arrest was determined by measuring the cell number stained by 5'-bromo-2'-deoxyuridine (BrdU) and 7-amino-actinomycin D (7-AAD). Treatment of cells with SBE resulted in a concentration- and time-dependent inhibition of growth and a G, phase arrest of the cell cycle. This effect was associated with a marked decrease in the protein expression of cyclin A, D1, D2, D3, and E and their activating partners, cyclin-dependent kinases (CDK) 2, 4, and 6 with concomitant upregulation of p21, cyclin-dependent kinase inhibitor. Downstream of the CDK inhibitory protein-CDK/cyclin cascade, SBE decreased phosphorylation level of retinoblastoma protein. SBE treatment also resulted in apoptosis evidenced by an increase of sub-G, phase cells, DNA fragmentation and degradation of the inhibitory protein for the caspase-activated deoxyribonuclease. The molecular mechanism during SBE-mediated growth inhibition in HL-60 cells may be due to modulation of the cell-cycle machinery and the induction of apoptosis. | - |
dc.language | English | - |
dc.publisher | PROFESSOR D A SPANDIDOS | - |
dc.title | Induction of G1 arrest and apoptosis by Scutellaria barbata in the human promyelocytic leukemia HL-60 cell line | - |
dc.type | Article | - |
dc.identifier.wosid | 000247492900017 | - |
dc.type.rims | ART | - |
dc.citation.volume | 20 | - |
dc.citation.issue | 1 | - |
dc.citation.beginningpage | 123 | - |
dc.citation.endingpage | 128 | - |
dc.citation.publicationname | INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE | - |
dc.contributor.localauthor | Park, Byung-Hyun | - |
dc.contributor.nonIdAuthor | Kim, Eun-Kyung | - |
dc.contributor.nonIdAuthor | Kwon, Kang-Beom | - |
dc.contributor.nonIdAuthor | Han, Mi-Jeong | - |
dc.contributor.nonIdAuthor | Song, Mi-Young | - |
dc.contributor.nonIdAuthor | Lee, Ji-Hyun | - |
dc.contributor.nonIdAuthor | Ko, Youn-Suk | - |
dc.contributor.nonIdAuthor | Shin, Byung-Cheal | - |
dc.contributor.nonIdAuthor | Yu, Jiahua | - |
dc.contributor.nonIdAuthor | Lee, Young-Rae | - |
dc.contributor.nonIdAuthor | Ryu, Do-Gon | - |
dc.contributor.nonIdAuthor | Park, Jin-Woo | - |
dc.description.isOpenAccess | N | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordAuthor | scutellaria barbata | - |
dc.subject.keywordAuthor | apoptosis | - |
dc.subject.keywordAuthor | cell cycle | - |
dc.subject.keywordAuthor | HL-60 cell | - |
dc.subject.keywordPlus | CYCLIN-DEPENDENT KINASES | - |
dc.subject.keywordPlus | CARCINOMA DU145 CELLS | - |
dc.subject.keywordPlus | RETINOBLASTOMA PROTEIN | - |
dc.subject.keywordPlus | SIGNALING PATHWAY | - |
dc.subject.keywordPlus | CDK INHIBITORS | - |
dc.subject.keywordPlus | CANCER | - |
dc.subject.keywordPlus | GROWTH | - |
dc.subject.keywordPlus | ACTIVATION | - |
dc.subject.keywordPlus | DEATH | - |
dc.subject.keywordPlus | DIFFERENTIATION | - |
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