DC Field | Value | Language |
---|---|---|
dc.contributor.author | Lee, Na-Ri | ko |
dc.contributor.author | Meng, Ruo Yu | ko |
dc.contributor.author | Rah, So-Young | ko |
dc.contributor.author | Jin, Hua | ko |
dc.contributor.author | Ray, Navin | ko |
dc.contributor.author | Kim, Seong-Hun | ko |
dc.contributor.author | Park, Byung Hyun | ko |
dc.contributor.author | Kim, Soo Mi | ko |
dc.date.accessioned | 2024-03-22T05:00:21Z | - |
dc.date.available | 2024-03-22T05:00:21Z | - |
dc.date.created | 2024-03-21 | - |
dc.date.created | 2024-03-21 | - |
dc.date.issued | 2020-12 | - |
dc.identifier.citation | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, v.21, no.24 | - |
dc.identifier.issn | 1661-6596 | - |
dc.identifier.uri | http://hdl.handle.net/10203/318686 | - |
dc.description.abstract | Ursolic acid (UA) possesses various pharmacological activities, such as antitumorigenic and anti-inflammatory effects. In the present study, we investigated the mechanisms underlying the effects of UA against esophageal squamous cell carcinoma (ESCC) (TE-8 cells and TE-12 cells). The cell viability assay showed that UA decreased the viability of ESCC in a dose-dependent manner. In the soft agar colony formation assay, the colony numbers and size were reduced in a dose-dependent manner after UA treatment. UA caused the accumulation of vacuoles and LC3 puncta, a marker of autophagosome, in a dose-dependent manner. Autophagy induction was confirmed by measuring the expression levels of LC3 and p62 protein in ESCC cells. UA increased LC3-II protein levels and decreased p62 levels in ESCC cells. When autophagy was hampered using 3-methyladenine (3-MA), the effect of UA on cell viability was reversed. UA also significantly inhibited protein kinase B (Akt) activation and increased p-Akt expression in a dose-dependent manner in ESCC cells. Accumulated LC3 puncta by UA was reversed after wortmannin treatment. LC3-II protein levels were also decreased after treatment with Akt inhibitor and wortmannin. Moreover, UA treatment increased cellular reactive oxygen species (ROS) levels in ESCC in a time- and dose-dependent manner. Diphenyleneiodonium (an ROS production inhibitor) blocked the ROS and UA induced accumulation of LC3-II levels in ESCC cells, suggesting that UA-induced cell death and autophagy are mediated by ROS. Therefore, our data indicate that UA inhibits the growth of ESCC cells by inducing ROS-dependent autophagy. | - |
dc.language | English | - |
dc.publisher | MDPI | - |
dc.title | Reactive Oxygen Species-Mediated Autophagy by Ursolic Acid Inhibits Growth and Metastasis of Esophageal Cancer Cells | - |
dc.type | Article | - |
dc.identifier.wosid | 000603385900001 | - |
dc.identifier.scopusid | 2-s2.0-85097548109 | - |
dc.type.rims | ART | - |
dc.citation.volume | 21 | - |
dc.citation.issue | 24 | - |
dc.citation.publicationname | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | - |
dc.identifier.doi | 10.3390/ijms21249409 | - |
dc.contributor.localauthor | Park, Byung Hyun | - |
dc.contributor.nonIdAuthor | Lee, Na-Ri | - |
dc.contributor.nonIdAuthor | Meng, Ruo Yu | - |
dc.contributor.nonIdAuthor | Rah, So-Young | - |
dc.contributor.nonIdAuthor | Jin, Hua | - |
dc.contributor.nonIdAuthor | Ray, Navin | - |
dc.contributor.nonIdAuthor | Kim, Seong-Hun | - |
dc.contributor.nonIdAuthor | Kim, Soo Mi | - |
dc.description.isOpenAccess | N | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordAuthor | ursolic acid | - |
dc.subject.keywordAuthor | esophageal squamous cell carcinoma | - |
dc.subject.keywordAuthor | anticancer | - |
dc.subject.keywordAuthor | autophagy | - |
dc.subject.keywordAuthor | reactive oxygen species | - |
dc.subject.keywordAuthor | cell death | - |
dc.subject.keywordPlus | COLORECTAL-CANCER | - |
dc.subject.keywordPlus | INDUCED APOPTOSIS | - |
dc.subject.keywordPlus | ACTIVATION | - |
dc.subject.keywordPlus | DEATH | - |
dc.subject.keywordPlus | PATHWAY | - |
dc.subject.keywordPlus | AKT | - |
dc.subject.keywordPlus | PROLIFERATION | - |
dc.subject.keywordPlus | PROMOTES | - |
dc.subject.keywordPlus | SUPPRESSION | - |
dc.subject.keywordPlus | METABOLISM | - |
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