Study on biologically active compounds isolated from streptomyces and echinomycin derivatives = 방선균으로부터 유도된 생리활성물질 및 이치노마이신 유도체에 대한 생리활성연구

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The current study is undertaken to investigate the new tumor cell growth inhibitors derived from Streptomyces. The producing organism was isolated from soil collected at the Wonju area (CHIAK mountain) in Kangwon-Do, Korea. The 800 microorganisms were tested in primary screening. Primary screening tests were performed using antimicrobial activity and tumor cell cytotoxicity in vitro. Cultured broth of microorganism was tested for antimicrobial activity against Staphylococcus aureus and for cytotoxicity against P 388 murin cell line. Finally, we chose the SK-302 which has a excellent antimicrobial activity and tumor cell cytotoxicity. The selected microorganism SK-302 belongs to Actinomycetes Streptomyces sp. based on morphological and cultural properties on various medium. The SK-302 was cultivated in large scale, lab and 300L pilot-scale fermentor, to prepare the sample. Active metabolites were isolateded from cultured broth by several isolation steps of extractions and column chromatography (silica column). Purification was carried out using the Prep-HPLC with ODS column and 1H-2H polystylene gel column. As a result, about 500 mg of pure SK-302B and 27 mg of pure SK-302C were obtained from cultured broth. Pure SK-302B and SK-302C were tested in vitro (3 LL, B 16 mouse tumor cell), in vivo (P 388, B 16, S-180 mouse tumor cell), in vitro (SK-MEL-2, HCT-15, SNU-1, KATO human tumor cell) tests, based on chemosensitivity tests (cytotoxicity and colony forming inhibition assay). Structural study of purified fractions were carried out several spectroscopic methods (2D FT-NMR and APCI, FAB MASS) and amino acid analysis. As a result, SK-302B (mol.wt 1101.3) is a cyclooctapeptide (essential amino acid: Ala, Ser, non-essential amino acid N-Me Cys, N-Me Val) with aromatic (quinoxaline) moiety. The candidate compound for antitumor agent, SK-302B, was finally identified as a known compound, echinomycin A (quinomycin A). Echinomycin and It``s synthetic analogue TANDEM i...
Advisors
Kim, Yong-Hae김용해
Description
한국과학기술원 : 화학과,
Publisher
한국과학기술원
Issue Date
1998
Identifier
144202/325007 / 000925564
Language
eng
Description

학위논문(박사) - 한국과학기술원 : 화학과, 1998.8, [ xii, 116 p. ]

Keywords

Sulfonium salt; Anti-cancer; Derivatives; Echinomycin; Sulfoxide; 술폭사이드; 술포니움염; 항암제; 유도체; 이치노마이신

URI
http://hdl.handle.net/10203/31485
Link
http://library.kaist.ac.kr/search/detail/view.do?bibCtrlNo=144202&flag=dissertation
Appears in Collection
CH-Theses_Ph.D.(박사논문)
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