This paper presents a cell adhesion measurement chip with multiple shear stress zones in a single microchannel. In the experimental study, we used epithelial-like H358 and epithelial/mesenchymal-like A549 lung cancer cells to measure cell adhesion on the collagen layer. The detachment rate of H358 cells is 2∼4 times higher than that of A549 cells in the shear stress range of 34∼60dyne/cm2. The present chip is applicable to the quantitative analysis of tumor cell-extracellular matrix (ECM) adhesion.