Acoustofluidic lysis of cancer cells and Raman spectrum profiling

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The lysis of cancer cells inside a sessile droplet was performed using traveling surface acoustic waves (SAWs) without any chemical reagents. Raman spectrum profiling was then carried out to explore detailed cell-derived data. The Rayleigh waves formed by an interdigital transducer were made to propagate along the surface of an LiNbO3 substrate. Polystyrene microparticles (PSMPs) were used to establish mechanical cell lysis effectively, and gold nanoparticles (AuNPs) were added to enhance the Raman signals from the lysed cells by SAWs. The lysis efficiency was evaluated according to the size and concentration of the PSMPs in experiments where the frequency was varied. Lysis occurred mainly by mechanical collision using PSMPs in a high-frequency domain, and the lysis efficiency was improved by increasing the application time and the energy density of the SAWs. Raman signals from the lysed cells were greatly enhanced by nanogaps formed by the AuNPs, which were evenly distributed irrespective of the SAWs through the frequency-independent behavior of the AuNPs. Finally, detailed Raman spectra of MDA-MB-231, malignant breast cancer cells, were acquired, and various organic matter-derived peaks were observed. The 95% confidence region for cells subjected to lysis was more widely distributed than that of cells not subjected to lysis. The proposed SAW platform is expected to facilitate the detection of small quantities and to be applied in biomedical applications. Acoustofluidic cancer cell lysis and particle mixing facilitate Raman spectrum profiling.
Publisher
ROYAL SOC CHEMISTRY
Issue Date
2023-09
Language
English
Article Type
Article
Citation

LAB ON A CHIP, v.23, no.18, pp.4117 - 4125

ISSN
1473-0197
DOI
10.1039/d3lc00550
URI
http://hdl.handle.net/10203/312885
Appears in Collection
ME-Journal Papers(저널논문)
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