DC Field | Value | Language |
---|---|---|
dc.contributor.author | Yoo, Jungmin | ko |
dc.contributor.author | Lee, Donghun | ko |
dc.contributor.author | Im, Hyeryeon | ko |
dc.contributor.author | Ji, Sangmi | ko |
dc.contributor.author | Oh, Sanghoon | ko |
dc.contributor.author | Shin, Minsang | ko |
dc.contributor.author | Park, Daeho | ko |
dc.contributor.author | Lee, Gwangrog | ko |
dc.date.accessioned | 2023-09-12T01:00:46Z | - |
dc.date.available | 2023-09-12T01:00:46Z | - |
dc.date.created | 2023-09-12 | - |
dc.date.issued | 2021-07 | - |
dc.identifier.citation | SCIENCE ADVANCES, v.7, no.29 | - |
dc.identifier.uri | http://hdl.handle.net/10203/312433 | - |
dc.description.abstract | During base excision repair, a transient single-stranded DNA (ssDNA) gap is produced at the apurinic/apyrimidinic (AP) site. Exonuclease III, capable of performing both AP endonuclease and exonuclease activity, are responsible for gap creation in bacteria. We used single-molecule fluorescence resonance energy transfer to examine the mechanism of gap creation. We found an AP site anchor-based mechanism by which the intrinsically distributive enzyme binds strongly to the AP site and becomes a processive enzyme, rapidly creating a gap and an associated transient ssDNA loop. The gap size is determined by the rigidity of the ssDNA loop and the duplex stability of the DNA and is limited to a few nucleotides to maintain genomic stability. When the 3' end is released from the AP endonuclease, polymerase I quickly initiates DNA synthesis and fills the gap. Our work provides previously unidentified insights into how a signal of DNA damage changes the enzymatic functions. | - |
dc.language | English | - |
dc.publisher | AMER ASSOC ADVANCEMENT SCIENCE | - |
dc.title | The mechanism of gap creation by a multifunctional nuclease during base excision repair | - |
dc.type | Article | - |
dc.identifier.wosid | 000672818200011 | - |
dc.identifier.scopusid | 2-s2.0-85110337563 | - |
dc.type.rims | ART | - |
dc.citation.volume | 7 | - |
dc.citation.issue | 29 | - |
dc.citation.publicationname | SCIENCE ADVANCES | - |
dc.identifier.doi | 10.1126/sciadv.abg0076 | - |
dc.contributor.localauthor | Lee, Gwangrog | - |
dc.contributor.nonIdAuthor | Yoo, Jungmin | - |
dc.contributor.nonIdAuthor | Lee, Donghun | - |
dc.contributor.nonIdAuthor | Im, Hyeryeon | - |
dc.contributor.nonIdAuthor | Ji, Sangmi | - |
dc.contributor.nonIdAuthor | Oh, Sanghoon | - |
dc.contributor.nonIdAuthor | Shin, Minsang | - |
dc.contributor.nonIdAuthor | Park, Daeho | - |
dc.description.isOpenAccess | N | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordPlus | COLI ENDONUCLEASE-IV | - |
dc.subject.keywordPlus | SINGLE-STRANDED-DNA | - |
dc.subject.keywordPlus | ESCHERICHIA-COLI | - |
dc.subject.keywordPlus | EXONUCLEASE-III | - |
dc.subject.keywordPlus | DEOXYRIBONUCLEIC-ACID | - |
dc.subject.keywordPlus | LAMBDA-EXONUCLEASE | - |
dc.subject.keywordPlus | POLYMERASE | - |
dc.subject.keywordPlus | COORDINATION | - |
dc.subject.keywordPlus | DEGRADATION | - |
dc.subject.keywordPlus | LANDSCAPE | - |
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