To support IoT ecosystem, the closest technology to date is RFID sensor networks. In this paper, we propose an outstanding algorithm of anti-collision protocol to improve the readability of multiple tags in RFID systems. The proposed reservation and time-divided responses based query tree algorithm guarantees 100% readability and faster tag identification performance. As a result by simulating based on the system in the EPC Class 1 Gen. 2, the performance of the proposed scheme is significantly improved compared to that of the enhanced schemes based on query tree algorithm.