DC Field | Value | Language |
---|---|---|
dc.contributor.author | Friedrichs, Jens | ko |
dc.contributor.author | Legate, Kyle R. | ko |
dc.contributor.author | Schubert, Rajib | ko |
dc.contributor.author | Bharadwaj, Mitasha | ko |
dc.contributor.author | Werner, Carsten | ko |
dc.contributor.author | Muellner, Daniel J. | ko |
dc.contributor.author | Benoit, Martin | ko |
dc.date.accessioned | 2023-08-01T01:01:23Z | - |
dc.date.available | 2023-08-01T01:01:23Z | - |
dc.date.created | 2023-08-01 | - |
dc.date.issued | 2013-04 | - |
dc.identifier.citation | METHODS, v.60, no.2, pp.169 - 178 | - |
dc.identifier.issn | 1046-2023 | - |
dc.identifier.uri | http://hdl.handle.net/10203/310971 | - |
dc.description.abstract | Quantitative analysis of cellular interactions with the extracellular environment is necessary to gain an understanding of how cells regulate adhesion in the development and maintenance of multicellular organisms, and how changes in cell adhesion contribute to diseases. We provide a practical guide to quantify the adhesive strength of living animal cells to various substrates using atomic force microscopy (AFM)-based single-cell force spectroscopy (SCFS). We describe how to control cell state and attachment to the AFM cantilever, how to functionalize supports for SCFS measurements, how to conduct cell adhesion measurements, and how to analyze and interpret the recorded SCFS data. This guide is intended to assist newcomers in the field to perform AFM-based SCFS measurements. (C) 2013 Elsevier Inc. All rights reserved. | - |
dc.language | English | - |
dc.publisher | ACADEMIC PRESS INC ELSEVIER SCIENCE | - |
dc.title | A practical guide to quantify cell adhesion using single-cell force spectroscopy | - |
dc.type | Article | - |
dc.identifier.wosid | 000320297700008 | - |
dc.identifier.scopusid | 2-s2.0-84878223037 | - |
dc.type.rims | ART | - |
dc.citation.volume | 60 | - |
dc.citation.issue | 2 | - |
dc.citation.beginningpage | 169 | - |
dc.citation.endingpage | 178 | - |
dc.citation.publicationname | METHODS | - |
dc.identifier.doi | 10.1016/j.ymeth.2013.01.006 | - |
dc.contributor.localauthor | Schubert, Rajib | - |
dc.contributor.nonIdAuthor | Friedrichs, Jens | - |
dc.contributor.nonIdAuthor | Legate, Kyle R. | - |
dc.contributor.nonIdAuthor | Bharadwaj, Mitasha | - |
dc.contributor.nonIdAuthor | Werner, Carsten | - |
dc.contributor.nonIdAuthor | Muellner, Daniel J. | - |
dc.contributor.nonIdAuthor | Benoit, Martin | - |
dc.description.isOpenAccess | N | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordAuthor | AFM | - |
dc.subject.keywordAuthor | Atomic force microscopy | - |
dc.subject.keywordAuthor | Cell-cell adhesion | - |
dc.subject.keywordAuthor | Cell adhesion molecules | - |
dc.subject.keywordAuthor | Cell surface receptor | - |
dc.subject.keywordAuthor | Extracellular matrix | - |
dc.subject.keywordAuthor | Membrane tethers | - |
dc.subject.keywordAuthor | Receptor-ligand interactions | - |
dc.subject.keywordAuthor | SCFS | - |
dc.subject.keywordAuthor | Single-cell force spectroscopy | - |
dc.subject.keywordPlus | ACTIN STRESS FIBERS | - |
dc.subject.keywordPlus | PLATE FLOW CHAMBER | - |
dc.subject.keywordPlus | TRACTION FORCES | - |
dc.subject.keywordPlus | ERYTHROCYTE-MEMBRANE | - |
dc.subject.keywordPlus | MOLECULAR ADHESION | - |
dc.subject.keywordPlus | GLOBAL ASSESSMENT | - |
dc.subject.keywordPlus | OPTICAL TWEEZERS | - |
dc.subject.keywordPlus | EXTENSIONAL FLOW | - |
dc.subject.keywordPlus | FOCAL ADHESIONS | - |
dc.subject.keywordPlus | ELASTIC TETHER | - |
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