We present the fabric sheet layers, functionalized with graphene oxide (GO) for the sensitive isolation of circulating tumor cells (CTCs). Compared to the previous microfluidic-based CTC detection devices, which require complicated and long fabrication processes, the fabric sheet layers are simply fabricated by conventional textile manufacturing; thus the device is mass producible at extremely low-cost. Furthermore, since the present layers are composed of polyester yarns, GO can be directly modified on the surface after only a half hour of surface modification preprocess with protein, bovine serum albumin (BSA); thus, it has boundless potential for bioapplications. Here we demonstrated the fabric sheet layers and their simple modification methods to achieve high sensitive CTC isolation.