Protocol to prepare mouse spinal cord for patch-clamp and histology experiments

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The spinal cord is a part of the central nervous system located within the spinal canal of the vertebrae. Here, we present a protocol to prepare mouse spinal cord sections for patch-clamp and histology experiments. We describe steps for isolating spinal cord from the spinal canal and obtaining acute slices for patch-clamp experiments. For histology experiments, we detail fixing spinal cord for cryosectioning and imaging. This protocol provides procedures to assess neuronal activity and protein expression of sympathetic preganglionic neurons. For complete details on the use and execution of this protocol, please refer to Ju et al.
Publisher
ELSEVIER
Issue Date
2023-06
Language
English
Article Type
Article
Citation

STAR PROTOCOLS, v.4, no.2

ISSN
2666-1667
DOI
10.1016/j.xpro.2023.102345
URI
http://hdl.handle.net/10203/310291
Appears in Collection
BS-Journal Papers(저널논문)
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