DC Field | Value | Language |
---|---|---|
dc.contributor.author | Cho, Jae Sung | ko |
dc.contributor.author | Yang, Dongsoo | ko |
dc.contributor.author | Prabowo, Cindy Pricilia Surya | ko |
dc.contributor.author | Ghiffary, Mohammad Rifqi | ko |
dc.contributor.author | Han, Taehee | ko |
dc.contributor.author | Choi, Kyeong Rok | ko |
dc.contributor.author | Moon, Cheon Woo | ko |
dc.contributor.author | Zhou, Hengrui | ko |
dc.contributor.author | Ryu, Jae Yong | ko |
dc.contributor.author | Kim, Hyun Uk | ko |
dc.contributor.author | Lee, Sang Yup | ko |
dc.date.accessioned | 2023-06-29T10:00:23Z | - |
dc.date.available | 2023-06-29T10:00:23Z | - |
dc.date.created | 2023-06-28 | - |
dc.date.issued | 2023-06-11 | - |
dc.identifier.citation | Metabolic Engineering 15 | - |
dc.identifier.uri | http://hdl.handle.net/10203/310144 | - |
dc.description.abstract | The use of synthetic sRNAs for knockdown of target genes at the translational level has been restricted to a limited number of bacteria. In this work, we report the development of a broad-host-range synthetic sRNA (BHR-sRNA) platform employing the RoxS scaffold and the Hfq chaperone from Bacillus subtilis. The BHR-sRNA platform was tested in 16 bacterial species including commensal, probiotic, pathogenic, and industrial bacteria. Using this platform, more than 50% of target gene knockdown was achieved in 12 bacteria. As a medical application, virulence factors in pathogens Staphylococcus epidermidis and Klebsiella pneumoniae were knocked down to mitigate their virulence-associated phenotypes. For metabolic engineering applications, the development of high-performance Corynebacterium glutamicum strains capable of producing valerolactam and methyl anthranilate as example bulk and fine chemicals, respectively, were demonstrated by combinatorial knockdown of target genes. To facilitate high-throughput colorimetric screening of indigoidine (natural colorant) overproducers, a genome-scale sRNA library covering 2,959 C. glutamicum genes was constructed. The BHR-sRNA platform has the potential to expedite the engineering of diverse bacteria of both industrial and medical interest. This work was further supported by the Development of Next-Generation Biorefinery Platform Technologies for Leading Bio-based Chemicals Industry Project (2022M3J5A1056072) and by the Development of Platform Technologies of Microbial Cell Factories for the Next-Generation Biorefineries Project (2022M3J5A1056117) from the National Research Foundation supported by the Korean Ministry of Science and ICT. | - |
dc.language | English | - |
dc.publisher | American Institute of Chemical Engineers (AIChE) | - |
dc.title | Development of synthetic sRNAs tool for targeted and high-throughput gene knockdown in diverse bacteria | - |
dc.type | Conference | - |
dc.type.rims | CONF | - |
dc.citation.publicationname | Metabolic Engineering 15 | - |
dc.identifier.conferencecountry | SI | - |
dc.identifier.conferencelocation | Marina Bay Sands, Singapore | - |
dc.contributor.localauthor | Kim, Hyun Uk | - |
dc.contributor.localauthor | Lee, Sang Yup | - |
dc.contributor.nonIdAuthor | Prabowo, Cindy Pricilia Surya | - |
dc.contributor.nonIdAuthor | Ghiffary, Mohammad Rifqi | - |
dc.contributor.nonIdAuthor | Han, Taehee | - |
dc.contributor.nonIdAuthor | Choi, Kyeong Rok | - |
dc.contributor.nonIdAuthor | Moon, Cheon Woo | - |
dc.contributor.nonIdAuthor | Zhou, Hengrui | - |
dc.contributor.nonIdAuthor | Ryu, Jae Yong | - |
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