DC Field | Value | Language |
---|---|---|
dc.contributor.advisor | Li, Sheng | - |
dc.contributor.advisor | 리섕 | - |
dc.contributor.author | Hong, Jee-Hyun | - |
dc.date.accessioned | 2023-06-23T19:31:35Z | - |
dc.date.available | 2023-06-23T19:31:35Z | - |
dc.date.issued | 2022 | - |
dc.identifier.uri | http://library.kaist.ac.kr/search/detail/view.do?bibCtrlNo=997529&flag=dissertation | en_US |
dc.identifier.uri | http://hdl.handle.net/10203/308862 | - |
dc.description | 학위논문(석사) - 한국과학기술원 : 생명화학공학과, 2022.2,[iv, 54 p. :] | - |
dc.description.abstract | Development of high fluorescence, target-specific organic dye system is crucial for biological detection and imaging fields. To overcome the limited applications of organic dyes, dye-graft-polymer system was developed. Reactive polymer, poly(pentafluorophenyl acrylate), was utilized as backbone to immobilize near-infrared region dye, IR775. Hydrophilicity of the polymer was adjusted by additional substitution with amino-2-propanol or polyethylene glycol. Biotinylation of the polymer added target-specific binding ability to dye-graft-polymer system to bind with streptavidin protein. Absorbance and fluorescence spectra were examined for synthesized dye-graft-polymers and the grafting density of IR775 was optimized to 3%. Then, selectivity of biotinylated dye-graft-polymer toward streptavidin coated materials was examined. Biotinylated dye-graft-polymer selectively bound to streptavidin with high fluorescence signal. Biotinylated dye-graft-polymer system is expected to be efficient fluorescence system with high fluorescence, target-specific binding ability, and adjustable characteristics. | - |
dc.language | eng | - |
dc.publisher | 한국과학기술원 | - |
dc.title | Development of biotinylated fluorescent dye-graft-poly(pentafluorophenyl acrylate) hybrid polymer | - |
dc.title.alternative | 바이오틴이 결합된 형광 염료-그래프트-폴리(펜타플루오로페닐 아크릴레이트) 하이브리드 고분자의 합성 | - |
dc.type | Thesis(Master) | - |
dc.identifier.CNRN | 325007 | - |
dc.description.department | 한국과학기술원 :생명화학공학과, | - |
dc.contributor.alternativeauthor | 홍지현 | - |
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