Development of nanozyme by resembling the cofactor structure of nature enzyme and its application

Abstract

Enzymes are among the most useful catalysts in our bodies and have a wide range of applications, but because they are formed of protein, it is difficult to mass-produce them, and they are unstable, which highlights a key cost limitation. In order to replace the enzyme, a lot of attention has recently been drawn to research on nanomaterials with enzyme-like activity (nanozyme). Due to low activity or a lack of development strategy, previous reported nanozyme have been difficult to use practically. In this thesis, first of all, a study was conducted to selectively increase peroxidase activity through a strategy to imitate the structure of cofactor, the actual active site of enzyme. Next, mesoporous cerium oxide was doped with Cobalt to develop nanozyme capable of immobilizing oxidative enzyme while having peroxidase-like activity in neutral pH. Finally, by imitating the cofactor of organophosphorus hydrolase (OPH), we developed a Zinc doped mesoporous cerium oxide with OPH-like activity, and proved that it is an effective strategy not only for peroxidase but also for the development of other enzyme mimicking nanozyme.