DC Field | Value | Language |
---|---|---|
dc.contributor.author | Cho, Jae Sung | ko |
dc.contributor.author | Yang, Dongsoo | ko |
dc.contributor.author | Prabowo, Cindy Pricilia Surya | ko |
dc.contributor.author | Ghiffary, Mohammad Rifqi | ko |
dc.contributor.author | Han, Taehee | ko |
dc.contributor.author | Choi, Kyeong Rok | ko |
dc.contributor.author | Moon, Cheonwoo | ko |
dc.contributor.author | Zhou, Hengrui | ko |
dc.contributor.author | Ryu, Jae Yong | ko |
dc.contributor.author | Kim, Hyun Uk | ko |
dc.contributor.author | Lee, Sang Yup | ko |
dc.date.accessioned | 2023-05-12T02:01:14Z | - |
dc.date.available | 2023-05-12T02:01:14Z | - |
dc.date.created | 2023-05-08 | - |
dc.date.created | 2023-05-08 | - |
dc.date.issued | 2023-04 | - |
dc.identifier.citation | NATURE COMMUNICATIONS, v.14, no.1 | - |
dc.identifier.issn | 2041-1723 | - |
dc.identifier.uri | http://hdl.handle.net/10203/306749 | - |
dc.description.abstract | Synthetic sRNAs allow knockdown of target genes at translational level, but have been restricted to a limited number of bacteria. Here, we report the development of a broad-host-range synthetic sRNA (BHR-sRNA) platform employing the RoxS scaffold and the Hfq chaperone from Bacillus subtilis. BHR-sRNA is tested in 16 bacterial species including commensal, probiotic, pathogenic, and industrial bacteria, with >50% of target gene knockdown achieved in 12 bacterial species. For medical applications, virulence factors in Staphylococcus epidermidis and Klebsiella pneumoniae are knocked down to mitigate their virulence-associated phenotypes. For metabolic engineering applications, high performance Corynebacterium glutamicum strains capable of producing valerolactam (bulk chemical) and methyl anthranilate (fine chemical) are developed by combinatorial knockdown of target genes. A genome-scale sRNA library covering 2959 C. glutamicum genes is constructed for high-throughput colorimetric screening of indigoidine (natural colorant) overproducers. The BHR-sRNA platform will expedite engineering of diverse bacteria of both industrial and medical interest. | - |
dc.language | English | - |
dc.publisher | NATURE PORTFOLIO | - |
dc.title | Targeted and high-throughput gene knockdown in diverse bacteria using synthetic sRNAs | - |
dc.type | Article | - |
dc.identifier.wosid | 001002170200025 | - |
dc.identifier.scopusid | 2-s2.0-85153687205 | - |
dc.type.rims | ART | - |
dc.citation.volume | 14 | - |
dc.citation.issue | 1 | - |
dc.citation.publicationname | NATURE COMMUNICATIONS | - |
dc.identifier.doi | 10.1038/s41467-023-38119-y | - |
dc.contributor.localauthor | Choi, Kyeong Rok | - |
dc.contributor.localauthor | Kim, Hyun Uk | - |
dc.contributor.localauthor | Lee, Sang Yup | - |
dc.description.isOpenAccess | N | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordPlus | STAPHYLOCOCCUS-EPIDERMIDIS | - |
dc.subject.keywordPlus | ESCHERICHIA-COLI | - |
dc.subject.keywordPlus | REGULATOR | - |
dc.subject.keywordPlus | HFQ | - |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.