DC Field | Value | Language |
---|---|---|
dc.contributor.author | Ma, Shi-Xun | ko |
dc.contributor.author | Seo, Bo Am | ko |
dc.contributor.author | Kim, Donghoon | ko |
dc.contributor.author | Xiong, Yulan | ko |
dc.contributor.author | Kwon, Seung-Hwan | ko |
dc.contributor.author | Brahmachari, Saurav | ko |
dc.contributor.author | Kim, Sangjune | ko |
dc.contributor.author | Kam, Tae-In | ko |
dc.contributor.author | Nirujogi, Raja Sekhar | ko |
dc.contributor.author | Kwon, Sang Ho | ko |
dc.contributor.author | Dawson, Valina L. | ko |
dc.contributor.author | Dawson, Ted M. | ko |
dc.contributor.author | Pandey, Akhilesh | ko |
dc.contributor.author | Na, Chan Hyun | ko |
dc.contributor.author | Ko, Han Seok | ko |
dc.date.accessioned | 2023-05-04T07:00:18Z | - |
dc.date.available | 2023-05-04T07:00:18Z | - |
dc.date.created | 2023-05-04 | - |
dc.date.created | 2023-05-04 | - |
dc.date.issued | 2021-07 | - |
dc.identifier.citation | JOURNAL OF PROTEOME RESEARCH, v.20, no.7, pp.3428 - 3443 | - |
dc.identifier.issn | 1535-3893 | - |
dc.identifier.uri | http://hdl.handle.net/10203/306616 | - |
dc.description.abstract | Parkinson's disease (PD) is the second most common neurodegenerative disorder that results in motor dysfunction and, eventually, cognitive impairment. alpha-Synuclein protein is known as a central protein to the pathophysiology of PD, but the underlying pathological mechanism still remains to be elucidated. In an effort to understand how alpha-synuclein underlies the pathology of PD, various PD mouse models with alpha-synuclein overexpression have been developed. However, systemic analysis of the brain proteome of those mouse models is lacking. In this study, we established two mouse models of PD by injecting alpha-synuclein preformed fibrils (PFF) or by inducing overexpression of human A53T alpha-synuclein to investigate common pathways in the two different types of the PD mouse models. For more accurate quantification of mouse brain proteome, the proteins were quantified using the method of stable isotope labeling with amino acids in mammals . We identified a total of 8355 proteins from the two mouse models; similar to 6800 and similar to 7200 proteins from alpha-synuclein PFF-injected mice and human A53T alpha-synuclein transgenic mice, respectively. Through pathway analysis of the differentially expressed proteins common to both PD mouse models, it was discovered that the complement and coagulation cascade pathways were enriched in the PD mice compared to control animals. Notably, a validation study demonstrated that complement component 3 (C3)-positive astrocytes were increased in the ventral midbrain of the intrastriatal alpha-synuclein PFF-injected mice and C3 secreted from astrocytes could induce the degeneration of dopaminergic neurons. This is the first study that highlights the significance of the complement and coagulation pathways in the pathogenesis of PD through proteome analyses with two sophisticated mouse models of PD. | - |
dc.language | English | - |
dc.publisher | AMER CHEMICAL SOC | - |
dc.title | Complement and Coagulation Cascades are Potentially Involved in Dopaminergic Neurodegeneration in alpha-Synuclein-Based Mouse Models of Parkinson's Disease | - |
dc.type | Article | - |
dc.identifier.wosid | 000670662100004 | - |
dc.identifier.scopusid | 2-s2.0-85108404054 | - |
dc.type.rims | ART | - |
dc.citation.volume | 20 | - |
dc.citation.issue | 7 | - |
dc.citation.beginningpage | 3428 | - |
dc.citation.endingpage | 3443 | - |
dc.citation.publicationname | JOURNAL OF PROTEOME RESEARCH | - |
dc.identifier.doi | 10.1021/acs.jproteome.0c01002 | - |
dc.contributor.localauthor | Kam, Tae-In | - |
dc.contributor.nonIdAuthor | Ma, Shi-Xun | - |
dc.contributor.nonIdAuthor | Seo, Bo Am | - |
dc.contributor.nonIdAuthor | Kim, Donghoon | - |
dc.contributor.nonIdAuthor | Xiong, Yulan | - |
dc.contributor.nonIdAuthor | Kwon, Seung-Hwan | - |
dc.contributor.nonIdAuthor | Brahmachari, Saurav | - |
dc.contributor.nonIdAuthor | Kim, Sangjune | - |
dc.contributor.nonIdAuthor | Nirujogi, Raja Sekhar | - |
dc.contributor.nonIdAuthor | Kwon, Sang Ho | - |
dc.contributor.nonIdAuthor | Dawson, Valina L. | - |
dc.contributor.nonIdAuthor | Dawson, Ted M. | - |
dc.contributor.nonIdAuthor | Pandey, Akhilesh | - |
dc.contributor.nonIdAuthor | Na, Chan Hyun | - |
dc.contributor.nonIdAuthor | Ko, Han Seok | - |
dc.description.isOpenAccess | N | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordAuthor | Parkinson&apos | - |
dc.subject.keywordAuthor | s disease | - |
dc.subject.keywordAuthor | stable isotope labeling with amino acids in mammals (SILAM) | - |
dc.subject.keywordAuthor | alpha-synuclein | - |
dc.subject.keywordAuthor | mass spectrometry | - |
dc.subject.keywordAuthor | complement and coagulation cascades | - |
dc.subject.keywordAuthor | complement component 3 (C3) | - |
dc.subject.keywordAuthor | alpha-synuclein preformed fibrils | - |
dc.subject.keywordAuthor | TetP-A53T alpha-synuclein transgenic mouse | - |
dc.subject.keywordAuthor | dopaminergic neurons | - |
dc.subject.keywordAuthor | astrocytes | - |
dc.subject.keywordPlus | BLOOD-BRAIN-BARRIER | - |
dc.subject.keywordPlus | ALZHEIMERS-DISEASE | - |
dc.subject.keywordPlus | FACTOR-H | - |
dc.subject.keywordPlus | LEWY BODIES | - |
dc.subject.keywordPlus | FIBRINOGEN | - |
dc.subject.keywordPlus | ASSOCIATION | - |
dc.subject.keywordPlus | DEPOSITION | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordPlus | CONVERTASE | - |
dc.subject.keywordPlus | BIOMARKER | - |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.