Dual probing of redox species, NAD(P)H and HOCl, with a benzo[a]phenoxazine based far red-emitting dye

Abstract

We report a benzo[a]phenoxazine-based far red-emitting probe which selectively senses reduced nicotinamide adenine dinucleotide (NADH) and its phosphate ester NADPH, the most indispensable coenzymes found in all living cells, with "turn-off" fluorescence response at 650 nm. The original probe can be regenerated quantitatively through oxidation using hypochlorous acid, a reactive oxygen species, which was accompanied by restored fluorescence at 650 nm. This probe was also applied for fluorescence imaging of NADH in HeLa cells. (C) 2018 Elsevier Ltd. All rights reserved.