DC Field | Value | Language |
---|---|---|
dc.contributor.author | Kang, Byoung-Hoon | ko |
dc.contributor.author | Jang, Kyung-Won | ko |
dc.contributor.author | Yu, Eun-Sil | ko |
dc.contributor.author | Na, Hamin | ko |
dc.contributor.author | Lee, Yun-Jae | ko |
dc.contributor.author | Ko, Woong-Yeol | ko |
dc.contributor.author | Bae, NamHo | ko |
dc.contributor.author | Rho, Donggee | ko |
dc.contributor.author | Jeong, Ki -Hun | ko |
dc.date.accessioned | 2023-04-26T02:00:27Z | - |
dc.date.available | 2023-04-26T02:00:27Z | - |
dc.date.created | 2023-04-17 | - |
dc.date.created | 2023-04-17 | - |
dc.date.created | 2023-04-17 | - |
dc.date.issued | 2023-04 | - |
dc.identifier.citation | ACS NANO, v.17, no.7, pp.6507 - 6518 | - |
dc.identifier.issn | 1936-0851 | - |
dc.identifier.uri | http://hdl.handle.net/10203/306390 | - |
dc.description.abstract | Point-of-care real-time reverse-transcription pol-ymerase chain reaction (RT-PCR) facilitates the widespread use of rapid, accurate, and cost-effective near-patient testing that is available to the public. Here, we report ultrafast plasmonic nucleic acid amplification and real-time quantification for decentralized molecular diagnostics. The plasmonic real-time RT-PCR system features an ultrafast plasmonic thermocycler (PTC), a disposable plastic-on-metal (PoM) cartridge, and an ultrathin microlens array fluorescence (MAF) microscope. The PTC provides ultrafast photothermal cycling under white-light-emitting diode illumination and precise temperature monitor -ing with an integrated resistance temperature detector. The PoM thin film cartridge allows rapid heat transfer as well as complete light blocking from the photothermal excitation source, resulting in real-time and highly efficient PCR quantification. Besides, the MAF microscope exhibits close-up and high-contrast fluorescence microscopic imaging. All of the systems were fully packaged in a palm size for point-of-care testing. The real-time RT-PCR system demonstrates the rapid diagnosis of coronavirus disease-19 RNA virus within 10 min and yields 95.6% of amplification efficiency, 96.6% of classification accuracy for preoperational test, and 91% of total percent agreement for clinical diagnostic test. The ultrafast and compact PCR system can decentralize point-of-care molecular diagnostic testing in primary care and developing countries. | - |
dc.language | English | - |
dc.publisher | AMER CHEMICAL SOC | - |
dc.title | Ultrafast Plasmonic Nucleic Acid Amplification and Real-Time Quantification for Decentralized Molecular Diagnostics | - |
dc.type | Article | - |
dc.identifier.wosid | 000959825900001 | - |
dc.identifier.scopusid | 2-s2.0-85151318875 | - |
dc.type.rims | ART | - |
dc.citation.volume | 17 | - |
dc.citation.issue | 7 | - |
dc.citation.beginningpage | 6507 | - |
dc.citation.endingpage | 6518 | - |
dc.citation.publicationname | ACS NANO | - |
dc.identifier.doi | 10.1021/acsnano.2c11831 | - |
dc.contributor.localauthor | Jeong, Ki -Hun | - |
dc.contributor.nonIdAuthor | Lee, Yun-Jae | - |
dc.contributor.nonIdAuthor | Ko, Woong-Yeol | - |
dc.contributor.nonIdAuthor | Bae, NamHo | - |
dc.contributor.nonIdAuthor | Rho, Donggee | - |
dc.description.isOpenAccess | N | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordAuthor | plasmonic PCR | - |
dc.subject.keywordAuthor | real-time PCR | - |
dc.subject.keywordAuthor | ultrafast molecular diagnostics | - |
dc.subject.keywordAuthor | point-of-care test | - |
dc.subject.keywordAuthor | photothermal effect | - |
dc.subject.keywordAuthor | SARS-CoV-2 | - |
dc.subject.keywordPlus | HAND-HELD | - |
dc.subject.keywordPlus | PCR | - |
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