DC Field | Value | Language |
---|---|---|
dc.contributor.author | Shong, Minho | ko |
dc.contributor.author | Kim, YJ | ko |
dc.contributor.author | Choi, YM | ko |
dc.contributor.author | Kwon, OY | ko |
dc.date.accessioned | 2023-04-14T03:00:22Z | - |
dc.date.available | 2023-04-14T03:00:22Z | - |
dc.date.created | 2023-04-12 | - |
dc.date.created | 2023-04-12 | - |
dc.date.issued | 1999-07 | - |
dc.identifier.citation | ZEITSCHRIFT FUR NATURFORSCHUNG SECTION C-A JOURNAL OF BIOSCIENCES, v.54, no.7-8, pp.578 - 582 | - |
dc.identifier.issn | 0939-5075 | - |
dc.identifier.uri | http://hdl.handle.net/10203/306262 | - |
dc.description.abstract | Differential display (DD) PCR (Liang and Pardee, 1992) is a recently described technique to identify genes whose expression has changed during a biological process. We used this method to detect genes thyroid stimulating hormone-dependently regulated in a rat thyroid cell line, because thyroid stimulating hormone (TSH) is the most important hormone for cell proliferation and differentiation including prehormonal proteins secretion in thyrocytes (Kim and Arvan, 1991; Kim and Arvan, 1993). Following DD-PCR experimentation, thyroid stimulating hormone -dependently regulated gene fragments of 15 species were obtained. The genes were used as molecular probes in Northern blot analysis and then sequenced. Two of the clones (#123 and #205) were up-regulated and two more (#107 and #111) were down-regulated thyroid stimulating hormone-dependently in the thyroid cells, as demonstrated by Northern blot analysis. Following partial sequencing, each of the clones #107, #111 and #205 were shown to be homologues of the apoptosis-related gene, aldolase A, and a-2 collagen (IV), respectively, while clone #123 showed no homology with known genes. These findings suggest that the four genes mentioned above may have an a important physiological function in the thyrocytes, which is thyroid stimulating hormone-dependently up-/down-regulated. | - |
dc.language | English | - |
dc.publisher | VERLAG Z NATURFORSCH | - |
dc.title | Identification of genes in a thyroid cell line regulated by thyroid-stimulating hormone (TSH) | - |
dc.type | Article | - |
dc.identifier.wosid | 000082309200019 | - |
dc.type.rims | ART | - |
dc.citation.volume | 54 | - |
dc.citation.issue | 7-8 | - |
dc.citation.beginningpage | 578 | - |
dc.citation.endingpage | 582 | - |
dc.citation.publicationname | ZEITSCHRIFT FUR NATURFORSCHUNG SECTION C-A JOURNAL OF BIOSCIENCES | - |
dc.contributor.localauthor | Shong, Minho | - |
dc.contributor.nonIdAuthor | Kim, YJ | - |
dc.contributor.nonIdAuthor | Choi, YM | - |
dc.contributor.nonIdAuthor | Kwon, OY | - |
dc.description.isOpenAccess | N | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordAuthor | differential display (DD) PCR | - |
dc.subject.keywordAuthor | thyroid stimulating hormone (TSH) | - |
dc.subject.keywordAuthor | rat thyrocytes (FRTL5 cells) | - |
dc.subject.keywordPlus | ENDOPLASMIC-RETICULUM | - |
dc.subject.keywordPlus | DIFFERENTIAL DISPLAY | - |
dc.subject.keywordPlus | MESSENGER-RNA | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordPlus | ALDOLASE | - |
dc.subject.keywordPlus | HYPOTHYROIDISM | - |
dc.subject.keywordPlus | COMPONENTS | - |
dc.subject.keywordPlus | TISSUE | - |
dc.subject.keywordPlus | GOITER | - |
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