DC Field | Value | Language |
---|---|---|
dc.contributor.author | Montani, Valeria | ko |
dc.contributor.author | Taniguchi, Shin-ichi | ko |
dc.contributor.author | Shong, Minho | ko |
dc.contributor.author | Suzuki, Koichi | ko |
dc.contributor.author | Ohmori, Masayuki | ko |
dc.contributor.author | Giuliani, Cesidio | ko |
dc.contributor.author | Napolitano, Giorgio | ko |
dc.contributor.author | Saji, Motoyasu | ko |
dc.contributor.author | Fiorentino, Bruno | ko |
dc.contributor.author | Reimold, Andreas M | ko |
dc.contributor.author | Ting, Jenny PY | ko |
dc.contributor.author | Kohn, Leonard D | ko |
dc.contributor.author | Singer, Dinah S | ko |
dc.date.accessioned | 2023-04-14T00:00:14Z | - |
dc.date.available | 2023-04-14T00:00:14Z | - |
dc.date.created | 2023-04-12 | - |
dc.date.created | 2023-04-12 | - |
dc.date.issued | 1998-01 | - |
dc.identifier.citation | ENDOCRINOLOGY, v.139, no.1, pp.280 - 289 | - |
dc.identifier.issn | 0013-7227 | - |
dc.identifier.uri | http://hdl.handle.net/10203/306224 | - |
dc.description.abstract | Aberrant expression of major histocompatibility complex (MHC) class II proteins on thyrocytes; which is associated with autoimmune thyroid disease, is mimicked by gamma-interferon (gamma-IFN). To define elements and factors that regulate class II gene expression in thyrocytes and that might be involved in aberrant expression, we have studied gamma-IFN-induced HLA-DR alpha gene expression in rat FRTL-5 thyroid cells. The present report shows that class II expression in FRTL-5 thyrocytes is positively regulated by the class II transactivator (CIITA), and that CIITA mimics the action of gamma-IFN. Thus, as is the case for gamma-IFN, several distinct and highly conserved elements on the 5'-flanking region of the HLA-DR alpha gene, the S, X-1, X-2, and Y boxes between -137 to -65 bp, are required for class II gene expression induced by pCIITA transfection in FRTL-5 thyroid cells. CIITA and gamma-IFN do not cause additive increases in HLA-DR alpha gene expression in FRTL-5 cells, consistent with the possibility that CIITA is an intermediate factor in the gamma-IFN pathway to increased class II gene expression. Additionally, gamma-IFN treatment of FRTL-5 cells induces an endogenous CIITA transcript; pCIITA transfection mimics the ability of gamma-IFN treatment of FRTL-5 thyroid cells to increase the formation of a specific and novel protein/DNA complex containing CBP, a coactivator of CRE binding proteins important for cAMP-induced gene expression; and the action of both gamma-IFN and CIITA to increase class II gene expression and increase complex formation is reduced by cotransfection of a thyroid Y box protein, which suppresses MHC class I gene expression in FRTL-5 thyroid cells and is a homolog of human YB-1, which suppresses MHC class II expression in human glioma cells. We conclude that CIITA and TSH receptor suppressor element binding protein-1 are components of the gamma-IFN-regulated transduction system which, respectively, increase or decrease class II gene expression in thyrocytes and may, therefore, be involved in aberrant class II expression associated with autoimmune thyroid disease. | - |
dc.language | English | - |
dc.publisher | ENDOCRINE SOC | - |
dc.title | Major histocompatibility class II HLA-DR alpha gene expression in thyrocytes: Counter regulation by the class II transactivator and the thyroid Y box protein | - |
dc.type | Article | - |
dc.identifier.wosid | 000071146300040 | - |
dc.type.rims | ART | - |
dc.citation.volume | 139 | - |
dc.citation.issue | 1 | - |
dc.citation.beginningpage | 280 | - |
dc.citation.endingpage | 289 | - |
dc.citation.publicationname | ENDOCRINOLOGY | - |
dc.identifier.doi | 10.1210/en.139.1.280 | - |
dc.contributor.localauthor | Shong, Minho | - |
dc.contributor.nonIdAuthor | Montani, Valeria | - |
dc.contributor.nonIdAuthor | Taniguchi, Shin-ichi | - |
dc.contributor.nonIdAuthor | Suzuki, Koichi | - |
dc.contributor.nonIdAuthor | Ohmori, Masayuki | - |
dc.contributor.nonIdAuthor | Giuliani, Cesidio | - |
dc.contributor.nonIdAuthor | Napolitano, Giorgio | - |
dc.contributor.nonIdAuthor | Saji, Motoyasu | - |
dc.contributor.nonIdAuthor | Fiorentino, Bruno | - |
dc.contributor.nonIdAuthor | Reimold, Andreas M | - |
dc.contributor.nonIdAuthor | Ting, Jenny PY | - |
dc.contributor.nonIdAuthor | Kohn, Leonard D | - |
dc.contributor.nonIdAuthor | Singer, Dinah S | - |
dc.description.isOpenAccess | N | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordPlus | THYROTROPIN RECEPTOR GENE | - |
dc.subject.keywordPlus | TRANSCRIPTION FACTORS | - |
dc.subject.keywordPlus | RESPONSE ELEMENT | - |
dc.subject.keywordPlus | BINDING-PROTEIN | - |
dc.subject.keywordPlus | MAMMALIAN-CELLS | - |
dc.subject.keywordPlus | MHC | - |
dc.subject.keywordPlus | COMPLEX | - |
dc.subject.keywordPlus | INTERFERON | - |
dc.subject.keywordPlus | PROMOTER | - |
dc.subject.keywordPlus | CIITA | - |
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