DC Field | Value | Language |
---|---|---|
dc.contributor.author | Park, Yun Yong | ko |
dc.contributor.author | Kim, Han Jong | ko |
dc.contributor.author | Kim, Joon Young | ko |
dc.contributor.author | Kim, Mi Young | ko |
dc.contributor.author | Song, Kwang Hoon | ko |
dc.contributor.author | Park, Ki Cheol | ko |
dc.contributor.author | Yu, Kang Yeol | ko |
dc.contributor.author | Shong, Minho | ko |
dc.contributor.author | Kim, Kyoung Hee | ko |
dc.contributor.author | Choi, Hueng Sik | ko |
dc.date.accessioned | 2023-04-13T07:00:08Z | - |
dc.date.available | 2023-04-13T07:00:08Z | - |
dc.date.created | 2023-04-12 | - |
dc.date.created | 2023-04-12 | - |
dc.date.issued | 2004-05 | - |
dc.identifier.citation | MOLECULAR ENDOCRINOLOGY, v.18, no.5, pp.1082 - 1095 | - |
dc.identifier.issn | 0888-8809 | - |
dc.identifier.uri | http://hdl.handle.net/10203/306205 | - |
dc.description.abstract | The orphan nuclear receptors small heterodimer partner (SHP) and dosage-sensitive sex-reversal adrenal hypoplasia congenital (AHC) critical region on the X chromosome gene 1 (DAX-1) contain extra amino acids between helices H6 and H7 of LBD, and here we investigated a possible role of these additional amino acids. Transient transfection assay demonstrated that, in contrast to wild type, in mutant SHP Delta128 - 139 deletion of 12 extra amino acids in H6-H7 failed to repress the transactivity of orphan nuclear receptors such as estrogen receptor-related receptor-gamma, hepatocyte nuclear factor 4alpha, and constitutive androstane receptor. Interestingly, yeast two-hybrid and glutathione-S-transferase pull-down assays demonstrated that wildtype and SHP Delta128-139 have similar abilities to interact with estrogen receptor-related receptor-gamma, hepatocyte nuclear factor 4alpha, and constitutive androstane receptor. Unexpectedly, in wild-type DAX-1 and mutant DAX-1 Delta338 - 362, deletion of 25 extra amino acids in H6-H7 had no significant difference in the interaction and repression of steroidogenic factor 1 transactivation. Mutant SHP that contains DAX-1 extra amino acids or polyalanine stretch in H6-H7 showed indistinguishable pattern of repression from wild-type SHP. Interestingly, the swapped SHP mutant with DAX-1 extra amino acids interacted with EID-1 (E1A-like inhibitor of differentiation 1), which is characterized as an SHP-interacting corepressor. However, interaction between SHP Delta128-139 and EID-1 was significantly diminished. Moreover, SHP-mediated repression of constitutive androstane receptor transactivation was significantly released by down-regulation of EID-1 expression with EID-1 small interfering RNA. The present study suggests that H6-H7 loop regions of SHP and DAX-1 play a different role in the repression of nuclear receptor transactivation. | - |
dc.language | English | - |
dc.publisher | OXFORD UNIV PRESS INC | - |
dc.title | Differential role of the loop region between helices H6 and H7 within the orphan nuclear receptors small heterodimer partner and DAX-1 | - |
dc.type | Article | - |
dc.identifier.wosid | 000221054400003 | - |
dc.identifier.scopusid | 2-s2.0-2342447392 | - |
dc.type.rims | ART | - |
dc.citation.volume | 18 | - |
dc.citation.issue | 5 | - |
dc.citation.beginningpage | 1082 | - |
dc.citation.endingpage | 1095 | - |
dc.citation.publicationname | MOLECULAR ENDOCRINOLOGY | - |
dc.identifier.doi | 10.1210/me.2003-0339 | - |
dc.contributor.localauthor | Shong, Minho | - |
dc.contributor.nonIdAuthor | Park, Yun Yong | - |
dc.contributor.nonIdAuthor | Kim, Han Jong | - |
dc.contributor.nonIdAuthor | Kim, Joon Young | - |
dc.contributor.nonIdAuthor | Kim, Mi Young | - |
dc.contributor.nonIdAuthor | Song, Kwang Hoon | - |
dc.contributor.nonIdAuthor | Park, Ki Cheol | - |
dc.contributor.nonIdAuthor | Yu, Kang Yeol | - |
dc.contributor.nonIdAuthor | Kim, Kyoung Hee | - |
dc.contributor.nonIdAuthor | Choi, Hueng Sik | - |
dc.description.isOpenAccess | N | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordPlus | ADRENAL HYPOPLASIA CONGENITA | - |
dc.subject.keywordPlus | ACTIVATED ESTROGEN-RECEPTORS | - |
dc.subject.keywordPlus | LIGAND-BINDING DOMAIN | - |
dc.subject.keywordPlus | LXXLL-RELATED MOTIFS | - |
dc.subject.keywordPlus | X-RECEPTOR | - |
dc.subject.keywordPlus | TRANSCRIPTIONAL INHIBITOR | - |
dc.subject.keywordPlus | ANDROGEN RECEPTOR | - |
dc.subject.keywordPlus | HORMONE-RECEPTOR | - |
dc.subject.keywordPlus | SHP | - |
dc.subject.keywordPlus | GENE | - |
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