DC Field | Value | Language |
---|---|---|
dc.contributor.author | Kang, Jin Ku | ko |
dc.contributor.author | Chang, Chang-Hyun | ko |
dc.contributor.author | Nam, Hyo Jung | ko |
dc.contributor.author | Kim, Sung-Kuk | ko |
dc.contributor.author | Ahn, Keun Jae | ko |
dc.contributor.author | Seok, Heon | ko |
dc.contributor.author | Park, Sang Joon | ko |
dc.contributor.author | Kang, Yoon Joong | ko |
dc.contributor.author | Jo, Young Suk | ko |
dc.contributor.author | Shong, Minho | ko |
dc.contributor.author | Kim, Ho | ko |
dc.date.accessioned | 2023-04-12T07:01:11Z | - |
dc.date.available | 2023-04-12T07:01:11Z | - |
dc.date.created | 2023-04-12 | - |
dc.date.created | 2023-04-12 | - |
dc.date.issued | 2011-02 | - |
dc.identifier.citation | CELLULAR ONCOLOGY, v.34, no.1, pp.11 - 21 | - |
dc.identifier.issn | 2211-3428 | - |
dc.identifier.uri | http://hdl.handle.net/10203/306172 | - |
dc.description.abstract | Background Phospholipase C-gamma 1 (PLC-gamma 1) is known to play a critical role in cell adhesion and migration and is highly expressed in metastatic tumors. In the current study, we found that cells transformed by PLC-gamma 1 overexpression (PLC-gamma 1 cells) exhibited a marked decrease in expression of the Epo receptor (EpoR). Here, we assessed the role of EpoR-dependent signaling pathways in PLC-gamma 1-dependent regulation of cell adhesion and migration. Methods Expression and phosphorylation of EpoR and its functional role in PLC-gamma 1 cells were evaluated by immunoblot analysis or cell adhesion assay. The mechanism for PLC-gamma 1-induced EpoR downregulation was analyzed by blockage of proteosomal degradation with MG132. EpoR expression was also confirmed in colorectal cancer tissues in which PLC-gamma 1 was highly expressed. Results EpoR was present on rat fibroblasts, where it functionally active and capable of increasing cell adhesion and migratory activity. However, PLC-gamma 1 cells significantly decreased the Epo-dependent effects via ubiquitination-proteosomal degradation of EpoR. A marked decrease of EpoR expression was confirmed in colorectal cancer tissues that showed high-level of PLC-gamma 1 expression. Conclusion The Epo/EpoR complex plays a critical role in the adhesion and migration of rat fibroblasts, and its functional inactivation is associated with PLC-gamma 1-dependent reduction of cell-matrix adhesion and this also affects cell migration. | - |
dc.language | English | - |
dc.publisher | SPRINGER | - |
dc.title | Downregulation of erythropoietin receptor by overexpression of phospholipase C-gamma 1 is critical for decrease on focal adhesion in transformed cells | - |
dc.type | Article | - |
dc.identifier.wosid | 000290206900003 | - |
dc.identifier.scopusid | 2-s2.0-80052527859 | - |
dc.type.rims | ART | - |
dc.citation.volume | 34 | - |
dc.citation.issue | 1 | - |
dc.citation.beginningpage | 11 | - |
dc.citation.endingpage | 21 | - |
dc.citation.publicationname | CELLULAR ONCOLOGY | - |
dc.identifier.doi | 10.1007/s13402-010-0001-9 | - |
dc.contributor.localauthor | Shong, Minho | - |
dc.contributor.nonIdAuthor | Kang, Jin Ku | - |
dc.contributor.nonIdAuthor | Chang, Chang-Hyun | - |
dc.contributor.nonIdAuthor | Nam, Hyo Jung | - |
dc.contributor.nonIdAuthor | Kim, Sung-Kuk | - |
dc.contributor.nonIdAuthor | Ahn, Keun Jae | - |
dc.contributor.nonIdAuthor | Seok, Heon | - |
dc.contributor.nonIdAuthor | Park, Sang Joon | - |
dc.contributor.nonIdAuthor | Kang, Yoon Joong | - |
dc.contributor.nonIdAuthor | Jo, Young Suk | - |
dc.contributor.nonIdAuthor | Kim, Ho | - |
dc.description.isOpenAccess | N | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordAuthor | Phospholipase C-gamma 1 (PLC-gamma 1) | - |
dc.subject.keywordAuthor | Erythropoietin (Epo) | - |
dc.subject.keywordAuthor | Erythropoietin Receptor (EpoR) | - |
dc.subject.keywordAuthor | Proteosomal protein degradation | - |
dc.subject.keywordAuthor | Cell adhesion and migration/metastasis | - |
dc.subject.keywordAuthor | Focal contact formation | - |
dc.subject.keywordAuthor | Paxillin | - |
dc.subject.keywordAuthor | Colorectal cancer | - |
dc.subject.keywordPlus | SURFACE EXPRESSION | - |
dc.subject.keywordPlus | ELEVATED CONTENT | - |
dc.subject.keywordPlus | CANCER-PATIENTS | - |
dc.subject.keywordPlus | C-GAMMA-1 | - |
dc.subject.keywordPlus | KINASE | - |
dc.subject.keywordPlus | PHOSPHORYLATION | - |
dc.subject.keywordPlus | DEGRADATION | - |
dc.subject.keywordPlus | ACTIVATION | - |
dc.subject.keywordPlus | LYSOSOMES | - |
dc.subject.keywordPlus | MIGRATION | - |
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