DC Field | Value | Language |
---|---|---|
dc.contributor.author | Lee, Jung Uee | ko |
dc.contributor.author | Huang, Songmei | ko |
dc.contributor.author | Lee, Min Hee | ko |
dc.contributor.author | Lee, Seong Eun | ko |
dc.contributor.author | Ryu, Min Jeong | ko |
dc.contributor.author | Kim, Soung Jung | ko |
dc.contributor.author | Kim, Yong Kyung | ko |
dc.contributor.author | Kim, Seul Young | ko |
dc.contributor.author | Joung, Kyong Hye | ko |
dc.contributor.author | Kim, Jin Man | ko |
dc.contributor.author | Shong, Minho | ko |
dc.contributor.author | Jo, Young Suk | ko |
dc.date.accessioned | 2023-04-12T07:00:28Z | - |
dc.date.available | 2023-04-12T07:00:28Z | - |
dc.date.created | 2023-04-12 | - |
dc.date.created | 2023-04-12 | - |
dc.date.issued | 2012-07 | - |
dc.identifier.citation | EUROPEAN JOURNAL OF ENDOCRINOLOGY, v.167, no.1, pp.93 - 101 | - |
dc.identifier.issn | 0804-4643 | - |
dc.identifier.uri | http://hdl.handle.net/10203/306162 | - |
dc.description.abstract | Objective: The genetic mutations causing the constitutive activation of MEK/ERK have been regarded as an initiating factor in papillary thyroid carcinoma (PTC). The ERK-specific dual specificity phosphatase 6 (DUSP6) is part of the ERK-dependent transcriptional output. Therefore, the coordinated regulation of the activities of ERK kinases and DUSP6 may need to be reestablished to make new balances in PTC. Methods: To investigate the role of DUSP6 in the regulation of ERK1/2 (MAPK3/1)-dependent transcription, 42 benign neoplasms and 167 PTCs were retrospectively analyzed by immunohistochemistry with dideoxy sequencing to detect BRAF(V600E) mutation. Results: The expressions of total ERK1/2, DUSP6, c-Fos (FOS), c-Myc (MYC), cyclin D1, and PCNA were markedly increased in PTC compared with those in benign neoplasms. However, phospho-ERK1/2 was detected in only eight (4.8%) cases out of 167 PTC samples. Unexpectedly, the staining intensity and nuclear localization of ERK1/2 were not affected by the presence or absence of the BRAF(V600E) mutation. However, the expressions of c-Fos and PCNA were elevated in BRAF(V600E)-positive PTC compared with those in BRAF(V600E)-negative PTC. Interestingly, the higher staining intensities of DUSP6 were associated with the level of total ERK1/2 expression (P=0.04) and with high-risk biological features such as age (P=0.05), tumor size (P=0.01), and extrathyroidal extension (linear by linear association, P=0.02). In addition, DUSP6 silencing significantly decreased the cell viability and migration rate of FRO cells. Conclusions: The coordinated upregulation of total ERK1/2 and its phosphatase, DUSP6, is related to bare detection of phospho-ERK1/2 in PTC regardless of BRAF(V600E) mutation status. A link between DUSP6 expression and high-risk features of PTC suggested that DUSP6 is an important independent factor affecting the signaling pathways in established PTC. | - |
dc.language | English | - |
dc.publisher | BIOSCIENTIFICA LTD | - |
dc.title | Dual specificity phosphatase 6 as a predictor of invasiveness in papillary thyroid cancer | - |
dc.type | Article | - |
dc.identifier.wosid | 000305811400012 | - |
dc.identifier.scopusid | 2-s2.0-84862743813 | - |
dc.type.rims | ART | - |
dc.citation.volume | 167 | - |
dc.citation.issue | 1 | - |
dc.citation.beginningpage | 93 | - |
dc.citation.endingpage | 101 | - |
dc.citation.publicationname | EUROPEAN JOURNAL OF ENDOCRINOLOGY | - |
dc.identifier.doi | 10.1530/EJE-12-0010 | - |
dc.contributor.localauthor | Shong, Minho | - |
dc.contributor.nonIdAuthor | Lee, Jung Uee | - |
dc.contributor.nonIdAuthor | Huang, Songmei | - |
dc.contributor.nonIdAuthor | Lee, Min Hee | - |
dc.contributor.nonIdAuthor | Lee, Seong Eun | - |
dc.contributor.nonIdAuthor | Ryu, Min Jeong | - |
dc.contributor.nonIdAuthor | Kim, Soung Jung | - |
dc.contributor.nonIdAuthor | Kim, Yong Kyung | - |
dc.contributor.nonIdAuthor | Kim, Seul Young | - |
dc.contributor.nonIdAuthor | Joung, Kyong Hye | - |
dc.contributor.nonIdAuthor | Kim, Jin Man | - |
dc.contributor.nonIdAuthor | Jo, Young Suk | - |
dc.description.isOpenAccess | N | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordPlus | BRAF V600E MUTATION | - |
dc.subject.keywordPlus | BRAF(V600E) MUTATION | - |
dc.subject.keywordPlus | KINASE PHOSPHATASES | - |
dc.subject.keywordPlus | FEEDBACK-REGULATION | - |
dc.subject.keywordPlus | SIGNALING PATHWAY | - |
dc.subject.keywordPlus | ACTIVATION | - |
dc.subject.keywordPlus | GENE | - |
dc.subject.keywordPlus | RAF | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordPlus | ASSOCIATION | - |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.