DC Field | Value | Language |
---|---|---|
dc.contributor.author | Shin, Yong Cheol | ko |
dc.contributor.author | Shin, Woojung | ko |
dc.contributor.author | Koh, Domin | ko |
dc.contributor.author | Wu, Alexander | ko |
dc.contributor.author | Ambrosini, Yoko M. | ko |
dc.contributor.author | Min, Soyoun | ko |
dc.contributor.author | Eckhardt, S. Gail | ko |
dc.contributor.author | Fleming, R. Y. Declan | ko |
dc.contributor.author | Kim, Seung | ko |
dc.contributor.author | Park, Sowon | ko |
dc.contributor.author | Koh, Hong | ko |
dc.contributor.author | Yoo, Tae Kyung | ko |
dc.contributor.author | Kim, Hyun Jung | ko |
dc.date.accessioned | 2023-03-17T02:00:36Z | - |
dc.date.available | 2023-03-17T02:00:36Z | - |
dc.date.created | 2023-03-17 | - |
dc.date.created | 2023-03-17 | - |
dc.date.created | 2023-03-17 | - |
dc.date.issued | 2020-07 | - |
dc.identifier.citation | MICROMACHINES, v.11, no.7 | - |
dc.identifier.issn | 2072-666X | - |
dc.identifier.uri | http://hdl.handle.net/10203/305651 | - |
dc.description.abstract | The regeneration of the mucosal interface of the human intestine is critical in the host-gut microbiome crosstalk associated with gastrointestinal diseases. The biopsy-derived intestinal organoids provide genetic information of patients with physiological cytodifferentiation. However, the enclosed lumen and static culture condition substantially limit the utility of patient-derived organoids for microbiome-associated disease modeling. Here, we report a patient-specific three-dimensional (3D) physiodynamic mucosal interface-on-a-chip (PMI Chip) that provides a microphysiological intestinal milieu under defined biomechanics. The real-time imaging and computational simulation of the PMI Chip verified the recapitulation of non-linear luminal and microvascular flow that simulates the hydrodynamics in a living human gut. The multiaxial deformations in a convoluted microchannel not only induced dynamic cell strains but also enhanced particle mixing in the lumen microchannel. Under this physiodynamic condition, an organoid-derived epithelium obtained from the patients diagnosed with Crohn's disease, ulcerative colitis, or colorectal cancer independently formed 3D epithelial layers with disease-specific differentiations. Moreover, co-culture with the human fecal microbiome in an anoxic-oxic interface resulted in the formation of stochastic microcolonies without a loss of epithelial barrier function. We envision that the patient-specific PMI Chip that conveys genetic, epigenetic, and environmental factors of individual patients will potentially demonstrate the pathophysiological dynamics and complex host-microbiome crosstalk to target a patient-specific disease modeling. | - |
dc.language | English | - |
dc.publisher | MDPI | - |
dc.title | Three-Dimensional Regeneration of Patient-Derived Intestinal Organoid Epithelium in a Physiodynamic Mucosal Interface-on-a-Chip | - |
dc.type | Article | - |
dc.identifier.wosid | 000554145000001 | - |
dc.identifier.scopusid | 2-s2.0-85088375766 | - |
dc.type.rims | ART | - |
dc.citation.volume | 11 | - |
dc.citation.issue | 7 | - |
dc.citation.publicationname | MICROMACHINES | - |
dc.identifier.doi | 10.3390/mi11070663 | - |
dc.contributor.localauthor | Shin, Woojung | - |
dc.contributor.nonIdAuthor | Shin, Yong Cheol | - |
dc.contributor.nonIdAuthor | Koh, Domin | - |
dc.contributor.nonIdAuthor | Wu, Alexander | - |
dc.contributor.nonIdAuthor | Ambrosini, Yoko M. | - |
dc.contributor.nonIdAuthor | Min, Soyoun | - |
dc.contributor.nonIdAuthor | Eckhardt, S. Gail | - |
dc.contributor.nonIdAuthor | Fleming, R. Y. Declan | - |
dc.contributor.nonIdAuthor | Kim, Seung | - |
dc.contributor.nonIdAuthor | Park, Sowon | - |
dc.contributor.nonIdAuthor | Koh, Hong | - |
dc.contributor.nonIdAuthor | Yoo, Tae Kyung | - |
dc.contributor.nonIdAuthor | Kim, Hyun Jung | - |
dc.description.isOpenAccess | N | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordAuthor | gut-on-a-chip | - |
dc.subject.keywordAuthor | organoid | - |
dc.subject.keywordAuthor | mucosal interface | - |
dc.subject.keywordAuthor | physiodynamic | - |
dc.subject.keywordAuthor | multiaxial deformation | - |
dc.subject.keywordAuthor | microbiome | - |
dc.subject.keywordAuthor | co-culture | - |
dc.subject.keywordAuthor | disease modeling | - |
dc.subject.keywordPlus | HUMAN COLON | - |
dc.subject.keywordPlus | GUT | - |
dc.subject.keywordPlus | SYSTEMS | - |
dc.subject.keywordPlus | FLOW | - |
dc.subject.keywordPlus | MICROBIOME | - |
dc.subject.keywordPlus | PARADIGMS | - |
dc.subject.keywordPlus | TRANSPORT | - |
dc.subject.keywordPlus | DIGESTA | - |
dc.subject.keywordPlus | EFFLUX | - |
dc.subject.keywordPlus | GROWTH | - |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.