DC Field | Value | Language |
---|---|---|
dc.contributor.author | Shin, Woojung | ko |
dc.contributor.author | Ambrosini, Yoko M. | ko |
dc.contributor.author | Shin, Yong Cheol | ko |
dc.contributor.author | Wu, Alexander | ko |
dc.contributor.author | Min, Soyoun | ko |
dc.contributor.author | Koh, Domin | ko |
dc.contributor.author | Park, Sowon | ko |
dc.contributor.author | Kim, Seung | ko |
dc.contributor.author | Koh, Hong | ko |
dc.contributor.author | Kim, Hyun Jung | ko |
dc.date.accessioned | 2023-03-17T02:00:32Z | - |
dc.date.available | 2023-03-17T02:00:32Z | - |
dc.date.created | 2023-03-17 | - |
dc.date.created | 2023-03-17 | - |
dc.date.issued | 2020-08 | - |
dc.identifier.citation | FRONTIERS IN MEDICAL TECHNOLOGY, v.2 | - |
dc.identifier.uri | http://hdl.handle.net/10203/305650 | - |
dc.description.abstract | Polydimethylsiloxane (PDMS) is a silicone polymer that has been predominantly used in a human organ-on-a-chip microphysiological system. The hydrophobic surface of a microfluidic channel made of PDMS often results in poor adhesion of the extracellular matrix (ECM) as well as cell attachment. The surface modification by plasma or UV/ozone treatment in a PDMS-based device produces a hydrophilic surface that allows robust ECM coating and the reproducible attachment of human intestinal immortalized cell lines. However, these surface-activating methods have not been successful in forming a monolayer of the biopsy-derived primary organoid epithelium. Several existing protocols to grow human intestinal organoid cells in a PDMS microchannel are not always reproducibly operative due to the limited information. Here, we report an optimized methodology that enables robust and reproducible attachment of the intestinal organoid epithelium in a PDMS-based gut-on-a-chip. Among several reported protocols, we optimized a method by performing polyethyleneimine-based surface functionalization followed by the glutaraldehyde cross linking to activate the PDMS surface. Moreover, we discovered that the post-functionalization step contributes to provide uniform ECM deposition that allows to produce a robust attachment of the dissociated intestinal organoid epithelium in a PDMS-based microdevice. We envision that our optimized protocol may disseminate an enabling methodology to advance the integration of human organotypic cultures in a human organ-on-a-chip for patient-specific disease modeling. | - |
dc.language | English | - |
dc.publisher | FRONTIERS | - |
dc.title | Robust Formation of an Epithelial Layer of Human Intestinal Organoids in a Polydimethylsiloxane-Based Gut-on-a-Chip Microdevice | - |
dc.type | Article | - |
dc.type.rims | ART | - |
dc.citation.volume | 2 | - |
dc.citation.publicationname | FRONTIERS IN MEDICAL TECHNOLOGY | - |
dc.identifier.doi | 10.3389/fmedt.2020.00002 | - |
dc.contributor.localauthor | Shin, Woojung | - |
dc.contributor.nonIdAuthor | Ambrosini, Yoko M. | - |
dc.contributor.nonIdAuthor | Shin, Yong Cheol | - |
dc.contributor.nonIdAuthor | Wu, Alexander | - |
dc.contributor.nonIdAuthor | Min, Soyoun | - |
dc.contributor.nonIdAuthor | Koh, Domin | - |
dc.contributor.nonIdAuthor | Park, Sowon | - |
dc.contributor.nonIdAuthor | Kim, Seung | - |
dc.contributor.nonIdAuthor | Koh, Hong | - |
dc.contributor.nonIdAuthor | Kim, Hyun Jung | - |
dc.description.isOpenAccess | N | - |
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