Turn-On Fluorescence Sensing of Oxygen with Dendrimer-Encapsulated Platinum Nanoparticles as Tunable Oxidase Mimics for Spatially Resolved Measurement of Oxygen Gradient in a Human Gut-on-a-Chip

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dc.contributor.authorLee, Hyeinko
dc.contributor.authorShin, Woojungko
dc.contributor.authorKim, Hyun Jungko
dc.contributor.authorKim, Joohoonko
dc.date.accessioned2023-03-17T02:00:20Z-
dc.date.available2023-03-17T02:00:20Z-
dc.date.created2023-03-17-
dc.date.created2023-03-17-
dc.date.created2023-03-17-
dc.date.issued2021-12-
dc.identifier.citationANALYTICAL CHEMISTRY, v.93, no.48, pp.16123 - 16132-
dc.identifier.issn0003-2700-
dc.identifier.urihttp://hdl.handle.net/10203/305647-
dc.description.abstractTurn-on type fluorescence sensing of O-2 is considered a promising approach to developing ways to measure O-2 in microenvironments with spatially distributed O-2 levels. As a class of nanomaterials with a high degree of control over composition and structure, dendrimer-encapsulated nanoparticles (DENs) are promising candidates to mimic biological enzymes. Here, we report a strategy to monitor spatially distributed O-2 across a three-dimensional (3D) human intestinal epithelial layer in a gut-on-a-chip in a turn-on fluorescence sensing manner. The strategy is based on the oxidase-mimetic activity of Pt DENs for catalytic oxidation of nonfluorescent Amplex Red to highly fluorescent resorufin in the presence of O-2. We synthesized Pt DENs using two different types of dendrimers (i.e., amine- terminated or hydroxyl-terminated generation 6 polyamidoamine (PAMAM) dendrimers) with six different Pt2+/dendrimer ratios (i.e., 55, 200, 220, 550, 880, and 1320). After clarifying the intrinsic oxidase-mimetic activity of Pt DENs, we determined tunable oxidase-mimetic activity of Pt DENs, especially with fine-tuning the ratios of the Pt precursor ions and dendrimers. Particularly, the optimal Pt DENs having a Pt2+/dendrimer ratio of 1320 exhibited an similar to 117-fold increase in the oxidase-mimetic activity for catalyzing the aerobic oxidation of Amplex Red to resorufin compared to one having a Pt2+/dendrimer ratio of 200. This study exemplified a simple yet effective approach for spatially resolved imaging of O-2 using metal nanoparticle-based oxidase mimics in microphysiological environments like a human gut-on-a-chip.-
dc.languageEnglish-
dc.publisherAMER CHEMICAL SOC-
dc.titleTurn-On Fluorescence Sensing of Oxygen with Dendrimer-Encapsulated Platinum Nanoparticles as Tunable Oxidase Mimics for Spatially Resolved Measurement of Oxygen Gradient in a Human Gut-on-a-Chip-
dc.typeArticle-
dc.identifier.wosid000753022600039-
dc.identifier.scopusid2-s2.0-85120376692-
dc.type.rimsART-
dc.citation.volume93-
dc.citation.issue48-
dc.citation.beginningpage16123-
dc.citation.endingpage16132-
dc.citation.publicationnameANALYTICAL CHEMISTRY-
dc.identifier.doi10.1021/acs.analchem.1c03891-
dc.contributor.localauthorShin, Woojung-
dc.contributor.nonIdAuthorLee, Hyein-
dc.contributor.nonIdAuthorKim, Hyun Jung-
dc.contributor.nonIdAuthorKim, Joohoon-
dc.description.isOpenAccessN-
dc.type.journalArticleArticle-
dc.subject.keywordPlusPEROXIDASE-CATALYZED OXIDATION-
dc.subject.keywordPlusAMPLEX RED ASSAY-
dc.subject.keywordPlusSINGLET OXYGEN-
dc.subject.keywordPlusRESORUFIN IMPLICATIONS-
dc.subject.keywordPlusPHOTOOXIDATION-
dc.subject.keywordPlusSUPEROXIDE-
dc.subject.keywordPlusSIZE-
dc.subject.keywordPlusACTIVATION-
dc.subject.keywordPlusGENERATION-
dc.subject.keywordPlusPALLADIUM-
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