DC Field | Value | Language |
---|---|---|
dc.contributor.author | Lee, Hyein | ko |
dc.contributor.author | Shin, Woojung | ko |
dc.contributor.author | Kim, Hyun Jung | ko |
dc.contributor.author | Kim, Joohoon | ko |
dc.date.accessioned | 2023-03-17T02:00:20Z | - |
dc.date.available | 2023-03-17T02:00:20Z | - |
dc.date.created | 2023-03-17 | - |
dc.date.created | 2023-03-17 | - |
dc.date.created | 2023-03-17 | - |
dc.date.issued | 2021-12 | - |
dc.identifier.citation | ANALYTICAL CHEMISTRY, v.93, no.48, pp.16123 - 16132 | - |
dc.identifier.issn | 0003-2700 | - |
dc.identifier.uri | http://hdl.handle.net/10203/305647 | - |
dc.description.abstract | Turn-on type fluorescence sensing of O-2 is considered a promising approach to developing ways to measure O-2 in microenvironments with spatially distributed O-2 levels. As a class of nanomaterials with a high degree of control over composition and structure, dendrimer-encapsulated nanoparticles (DENs) are promising candidates to mimic biological enzymes. Here, we report a strategy to monitor spatially distributed O-2 across a three-dimensional (3D) human intestinal epithelial layer in a gut-on-a-chip in a turn-on fluorescence sensing manner. The strategy is based on the oxidase-mimetic activity of Pt DENs for catalytic oxidation of nonfluorescent Amplex Red to highly fluorescent resorufin in the presence of O-2. We synthesized Pt DENs using two different types of dendrimers (i.e., amine- terminated or hydroxyl-terminated generation 6 polyamidoamine (PAMAM) dendrimers) with six different Pt2+/dendrimer ratios (i.e., 55, 200, 220, 550, 880, and 1320). After clarifying the intrinsic oxidase-mimetic activity of Pt DENs, we determined tunable oxidase-mimetic activity of Pt DENs, especially with fine-tuning the ratios of the Pt precursor ions and dendrimers. Particularly, the optimal Pt DENs having a Pt2+/dendrimer ratio of 1320 exhibited an similar to 117-fold increase in the oxidase-mimetic activity for catalyzing the aerobic oxidation of Amplex Red to resorufin compared to one having a Pt2+/dendrimer ratio of 200. This study exemplified a simple yet effective approach for spatially resolved imaging of O-2 using metal nanoparticle-based oxidase mimics in microphysiological environments like a human gut-on-a-chip. | - |
dc.language | English | - |
dc.publisher | AMER CHEMICAL SOC | - |
dc.title | Turn-On Fluorescence Sensing of Oxygen with Dendrimer-Encapsulated Platinum Nanoparticles as Tunable Oxidase Mimics for Spatially Resolved Measurement of Oxygen Gradient in a Human Gut-on-a-Chip | - |
dc.type | Article | - |
dc.identifier.wosid | 000753022600039 | - |
dc.identifier.scopusid | 2-s2.0-85120376692 | - |
dc.type.rims | ART | - |
dc.citation.volume | 93 | - |
dc.citation.issue | 48 | - |
dc.citation.beginningpage | 16123 | - |
dc.citation.endingpage | 16132 | - |
dc.citation.publicationname | ANALYTICAL CHEMISTRY | - |
dc.identifier.doi | 10.1021/acs.analchem.1c03891 | - |
dc.contributor.localauthor | Shin, Woojung | - |
dc.contributor.nonIdAuthor | Lee, Hyein | - |
dc.contributor.nonIdAuthor | Kim, Hyun Jung | - |
dc.contributor.nonIdAuthor | Kim, Joohoon | - |
dc.description.isOpenAccess | N | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordPlus | PEROXIDASE-CATALYZED OXIDATION | - |
dc.subject.keywordPlus | AMPLEX RED ASSAY | - |
dc.subject.keywordPlus | SINGLET OXYGEN | - |
dc.subject.keywordPlus | RESORUFIN IMPLICATIONS | - |
dc.subject.keywordPlus | PHOTOOXIDATION | - |
dc.subject.keywordPlus | SUPEROXIDE | - |
dc.subject.keywordPlus | SIZE | - |
dc.subject.keywordPlus | ACTIVATION | - |
dc.subject.keywordPlus | GENERATION | - |
dc.subject.keywordPlus | PALLADIUM | - |
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