DC Field | Value | Language |
---|---|---|
dc.contributor.author | Yim, Sung Sun | ko |
dc.contributor.author | Choi, Jae Woong | ko |
dc.contributor.author | Lee, Yong Jae | ko |
dc.contributor.author | Jeong, Ki Jun | ko |
dc.date.accessioned | 2023-03-13T06:00:48Z | - |
dc.date.available | 2023-03-13T06:00:48Z | - |
dc.date.created | 2023-03-13 | - |
dc.date.created | 2023-03-13 | - |
dc.date.issued | 2023-02 | - |
dc.identifier.citation | MICROBIAL CELL FACTORIES, v.22, no.1 | - |
dc.identifier.issn | 1475-2859 | - |
dc.identifier.uri | http://hdl.handle.net/10203/305582 | - |
dc.description.abstract | BackgroundThe disposal of plastic waste is a major environmental challenge. With recent advances in microbial genetic and metabolic engineering technologies, microbial polyhydroxyalkanoates (PHAs) are being used as next-generation biomaterials to replace petroleum-based synthetic plastics in a sustainable future. However, the relatively high production cost of bioprocesses hinders the production and application of microbial PHAs on an industrial scale.ResultsHere, we describe a rapid strategy to rewire metabolic networks in an industrial microorganism, Corynebacterium glutamicum, for the enhanced production of poly(3-hydroxybutyrate) (PHB). A three-gene PHB biosynthetic pathway in Rasltonia eutropha was refactored for high-level gene expression. A fluorescence-based quantification assay for cellular PHB content using BODIPY was devised for the rapid fluorescence-activated cell sorting (FACS)-based screening of a large combinatorial metabolic network library constructed in C. glutamicum. Rewiring metabolic networks across the central carbon metabolism enabled highly efficient production of PHB up to 29% of dry cell weight with the highest cellular PHB productivity ever reported in C. glutamicum using a sole carbon source.ConclusionsWe successfully constructed a heterologous PHB biosynthetic pathway and rapidly optimized metabolic networks across central metabolism in C. glutamicum for enhanced production of PHB using glucose or fructose as a sole carbon source in minimal media. We expect that this FACS-based metabolic rewiring framework will accelerate strain engineering processes for the production of diverse biochemicals and biopolymers. | - |
dc.language | English | - |
dc.publisher | BMC | - |
dc.title | Rapid combinatorial rewiring of metabolic networks for enhanced poly(3-hydroxybutyrate) production in Corynebacterium glutamicum | - |
dc.type | Article | - |
dc.identifier.wosid | 000935867000001 | - |
dc.identifier.scopusid | 2-s2.0-85148378323 | - |
dc.type.rims | ART | - |
dc.citation.volume | 22 | - |
dc.citation.issue | 1 | - |
dc.citation.publicationname | MICROBIAL CELL FACTORIES | - |
dc.identifier.doi | 10.1186/s12934-023-02037-x | - |
dc.contributor.localauthor | Yim, Sung Sun | - |
dc.contributor.localauthor | Jeong, Ki Jun | - |
dc.contributor.nonIdAuthor | Choi, Jae Woong | - |
dc.contributor.nonIdAuthor | Lee, Yong Jae | - |
dc.description.isOpenAccess | N | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordAuthor | Corynebacterium glutamicum | - |
dc.subject.keywordAuthor | Metabolic engineering | - |
dc.subject.keywordAuthor | Combinatorial optimization | - |
dc.subject.keywordAuthor | Poly(3-hydroxybutyrate) | - |
dc.subject.keywordPlus | ESCHERICHIA-COLI | - |
dc.subject.keywordPlus | GENE-EXPRESSION | - |
dc.subject.keywordPlus | MALIC ENZYME | - |
dc.subject.keywordPlus | TCA CYCLE | - |
dc.subject.keywordPlus | PLATFORM | - |
dc.subject.keywordPlus | POLYHYDROXYALKANOATES | - |
dc.subject.keywordPlus | ACCUMULATION | - |
dc.subject.keywordPlus | OPTIMIZATION | - |
dc.subject.keywordPlus | PROTEINS | - |
dc.subject.keywordPlus | PATHWAY | - |
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