DC Field | Value | Language |
---|---|---|
dc.contributor.author | Kim, Hong-Sik | ko |
dc.contributor.author | Bae, Jin-Ho | ko |
dc.contributor.author | Kim, Gijeong | ko |
dc.contributor.author | Song, Ji-Joon | ko |
dc.contributor.author | Kim, Hak-Sung | ko |
dc.date.accessioned | 2023-03-13T03:00:17Z | - |
dc.date.available | 2023-03-13T03:00:17Z | - |
dc.date.created | 2023-03-13 | - |
dc.date.created | 2023-03-13 | - |
dc.date.created | 2023-03-13 | - |
dc.date.issued | 2023-02 | - |
dc.identifier.citation | SMALL, v.19, no.8 | - |
dc.identifier.issn | 1613-6810 | - |
dc.identifier.uri | http://hdl.handle.net/10203/305562 | - |
dc.description.abstract | Protein assemblies have drawn much attention as platforms for biomedical applications, including gene/drug delivery and vaccine, due to biocompatibility and functional diversity. Here, the construction and functionalization of a protein assembly composed of human clathrin heavy chain and light chain for a targeted protein delivery, is presented. The clathrin heavy and light chains are redesigned and associated with each other, and the resulting triskelion unit further self-assembled into a clathrin assembly with the size of about 28 nm in diameter. The clathrin assembly is dual-functionalized with a protein cargo and a targeting moiety using two different orthogonal protein-ligand pairs through one-pot reaction. The functionalized clathrin assembly exhibits about a 900-fold decreased K-D value for a cell-surface target due to avidity compared to a native targeting moiety. The utility of the clathrin assembly is demonstrated by an efficient delivery of a protein cargo into tumor cells in a target-specific manner, resulting in a strong cytotoxic effect. The present approach can be used in the creation of protein assemblies with multimodality. | - |
dc.language | English | - |
dc.publisher | WILEY-V C H VERLAG GMBH | - |
dc.title | Construction and Functionalization of a Clathrin Assembly for a Targeted Protein Delivery | - |
dc.type | Article | - |
dc.identifier.wosid | 000937012200026 | - |
dc.identifier.scopusid | 2-s2.0-85143394514 | - |
dc.type.rims | ART | - |
dc.citation.volume | 19 | - |
dc.citation.issue | 8 | - |
dc.citation.publicationname | SMALL | - |
dc.identifier.doi | 10.1002/smll.202204620 | - |
dc.contributor.localauthor | Song, Ji-Joon | - |
dc.contributor.localauthor | Kim, Hak-Sung | - |
dc.description.isOpenAccess | N | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordAuthor | avidity | - |
dc.subject.keywordAuthor | clathrin | - |
dc.subject.keywordAuthor | functionalization | - |
dc.subject.keywordAuthor | protein assembly | - |
dc.subject.keywordAuthor | protein delivery | - |
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