In this work, pure ZnZrO2 and chitosan supported (ZnZrO2/CS) nanocomposite have been synthesized at low coast by hydrothermal method. FT-IR, Micro Raman, PXRD, HR-SEM-EDAX, HR-TEM, AFM, BET and XPS were used to analyze the structural and morphological properties of the fabricated nanocomposites. The fabricated ZnZrO2 and ZnZrO2/CS nanocomposites were measured for their electrocatalytic activity towards glucose and hydrogen peroxide determinations. The ZnZrO2/CS sensor exhibited wide detection range (5 mu M to 5.85 mM), high sensitivity (6.78 mu A mM-1 cm-2), LOD (2.31 mu M), and long-term stability for glucose detection in alkaline solution. Also, as a multifunctional electrochemical sensor, ZnZrO2/CS sensor exhibits excellent sensing ability towards hydrogen peroxide, with a wide dynamic range (20 mu M to 6.85 mM), a high sensitivity (2.22 mu A mM-1 cm-2), and a LOD (2.08 mu M) (S/N = 3). The electrochemical measurement shows that the ZnZrO2/CS sensor has excellent catalytic activity and a much LOD than ZnZrO2. The modified electrode showed excellent anti inter-ference nature. Furthermore, this ZnZrO2/CS electrode was used to detection of glucose and H2O2 in human blood serum and HeLa cells respectively.