DC Field | Value | Language |
---|---|---|
dc.contributor.author | Li, Yan | ko |
dc.contributor.author | Kim, Hansol | ko |
dc.contributor.author | Ju, Yong | ko |
dc.contributor.author | Park, Yeonkyung | ko |
dc.contributor.author | Kang, Taejoon | ko |
dc.contributor.author | Yong, Dongeun | ko |
dc.contributor.author | Park, Hyun Gyu | ko |
dc.date.accessioned | 2023-01-03T01:00:21Z | - |
dc.date.available | 2023-01-03T01:00:21Z | - |
dc.date.created | 2023-01-02 | - |
dc.date.created | 2023-01-02 | - |
dc.date.issued | 2022-12 | - |
dc.identifier.citation | ANALYTICAL CHEMISTRY, v.94, no.50, pp.17448 - 17455 | - |
dc.identifier.issn | 0003-2700 | - |
dc.identifier.uri | http://hdl.handle.net/10203/303889 | - |
dc.description.abstract | The outbreak of the novel coronavirus disease 2019 (COVID-19) pandemic induced by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has caused millions of fatalities all over the world. Unquestionably, the effective and timely testing for infected individuals is the most imperative for the prevention of the ongoing pandemic. Herein, a new method was established for detecting SARS-CoV-2 based on the self-priming hairpin-utilized isothermal amplification of the G-rich sequence (SHIAG). In this strategy, the target RNA binding to the hairpin probe (HP) was uniquely devised to lead to the self-priming-mediated extension followed by the continuously repeated nicking and extension reactions, consequently generating abundant G-rich sequences from the intended reaction capable of producing fluorescence signals upon specifically interacting with thioflavin T (ThT). Based on the unique isothermal design concept, we successfully identified SARS-CoV-2 genomic RNA (gRNA) as low as 0.19 fM with excellent selectivity by applying only a single HP and further verified its practical diagnostic capability by reliably testing a total of 100 clinical specimens for COVID-19 with 100% clinical sensitivity and specificity. This study would provide notable insights into the design and evolution of new isothermal strategies for the sensitive and facile detection of SARS-CoV-2 under resource constraints. | - |
dc.language | English | - |
dc.publisher | AMER CHEMICAL SOC | - |
dc.title | Ultrasensitive Isothermal Detection of SARS-CoV-2 Based on Self-Priming Hairpin-Utilized Amplification of the G-Rich Sequence | - |
dc.type | Article | - |
dc.identifier.wosid | 000894955100001 | - |
dc.identifier.scopusid | 2-s2.0-85143849636 | - |
dc.type.rims | ART | - |
dc.citation.volume | 94 | - |
dc.citation.issue | 50 | - |
dc.citation.beginningpage | 17448 | - |
dc.citation.endingpage | 17455 | - |
dc.citation.publicationname | ANALYTICAL CHEMISTRY | - |
dc.identifier.doi | 10.1021/acs.analchem.2c03442 | - |
dc.contributor.localauthor | Park, Hyun Gyu | - |
dc.contributor.nonIdAuthor | Li, Yan | - |
dc.contributor.nonIdAuthor | Kang, Taejoon | - |
dc.contributor.nonIdAuthor | Yong, Dongeun | - |
dc.description.isOpenAccess | N | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordPlus | IN-VITRO AMPLIFICATION | - |
dc.subject.keywordPlus | DNA | - |
dc.subject.keywordPlus | COVID-19 | - |
dc.subject.keywordPlus | LAMP | - |
dc.subject.keywordPlus | OMICRON | - |
dc.subject.keywordPlus | ASSAY | - |
dc.subject.keywordPlus | BAD | - |
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