DC Field | Value | Language |
---|---|---|
dc.contributor.author | Park, Seung Eun | ko |
dc.contributor.author | Lee, Dongeun | ko |
dc.contributor.author | Jeong, Jae Woong | ko |
dc.contributor.author | Lee, Su-Hyung | ko |
dc.contributor.author | Park, Seung Ju | ko |
dc.contributor.author | Ryu, Jaeseung | ko |
dc.contributor.author | Oh, Se Kyu | ko |
dc.contributor.author | Yang, Hanseul | ko |
dc.contributor.author | Fang, Sungsoon | ko |
dc.contributor.author | Kim, Seyun | ko |
dc.date.accessioned | 2022-12-23T02:01:05Z | - |
dc.date.available | 2022-12-23T02:01:05Z | - |
dc.date.created | 2022-12-23 | - |
dc.date.created | 2022-12-23 | - |
dc.date.created | 2022-12-23 | - |
dc.date.created | 2022-12-23 | - |
dc.date.created | 2022-12-23 | - |
dc.date.issued | 2022-08 | - |
dc.identifier.citation | CELLULAR AND MOLECULAR GASTROENTEROLOGY AND HEPATOLOGY, v.14, no.6, pp.1235 - 1256 | - |
dc.identifier.issn | 2352-345X | - |
dc.identifier.uri | http://hdl.handle.net/10203/303613 | - |
dc.description.abstract | BACKGROUND & AIMS: Inositol polyphosphate multikinase (IPMK), an essential enzyme for inositol phosphate metabolism, has been known to mediate major biological events such as growth. Recent studies have identified single-nucleotide polymorphisms in the IPMK gene associated with inflammatory bowel disease predisposition. Therefore, we aimed to investigate the functional significance of IPMK in gut epithelium. METHODS: We generated intestinal epithelial cell (IEC)-specific Ipmk knockout (IPMKΔIEC) mice, and assessed their vulnerability against dextran sulfate sodium-induced experimental colitis. Both bulk and single-cell RNA sequencing were performed to analyze IPMK-deficient colonic epithelial cells and colonic tuft cells. RESULTS: Although IPMKΔIEC mice developed normally and showed no intestinal abnormalities during homeostasis, Ipmk deletion aggravated dextran sulfate sodium-induced colitis, with higher clinical colitis scores, and increased epithelial barrier permeability. Surprisingly, Ipmk deletion led to a significant decrease in the number of tuft cells without influencing other IECs. Single-cell RNA sequencing of mouse colonic tuft cells showed 3 distinct populations of tuft cells, and further showed that a transcriptionally inactive population was expanded markedly in IPMKΔIEC mice, while neuronal-related cells were relatively decreased. CONCLUSIONS: Cholinergic output from tuft cells is known to be critical for the restoration of intestinal architecture upon damage, supporting that tuft cell-defective IPMKΔIEC mice are more prone to colitis. Thus, intestinal epithelial IPMK is a critical regulator of colonic integrity and tissue regeneration by determining tuft cell homeostasis and affecting cholinergic output. Copyright © 2022 The Authors. Published by Elsevier Inc. All rights reserved. | - |
dc.language | English | - |
dc.publisher | ELSEVIER INC | - |
dc.title | Gut Epithelial Inositol Polyphosphate Multikinase Alleviates Experimental Colitis via Governing Tuft Cell Homeostasis | - |
dc.type | Article | - |
dc.identifier.wosid | 000898437900004 | - |
dc.identifier.scopusid | 2-s2.0-85141113798 | - |
dc.type.rims | ART | - |
dc.citation.volume | 14 | - |
dc.citation.issue | 6 | - |
dc.citation.beginningpage | 1235 | - |
dc.citation.endingpage | 1256 | - |
dc.citation.publicationname | CELLULAR AND MOLECULAR GASTROENTEROLOGY AND HEPATOLOGY | - |
dc.identifier.doi | 10.1016/j.jcmgh.2022.08.004 | - |
dc.contributor.localauthor | Yang, Hanseul | - |
dc.contributor.localauthor | Kim, Seyun | - |
dc.contributor.nonIdAuthor | Jeong, Jae Woong | - |
dc.contributor.nonIdAuthor | Lee, Su-Hyung | - |
dc.contributor.nonIdAuthor | Oh, Se Kyu | - |
dc.contributor.nonIdAuthor | Fang, Sungsoon | - |
dc.description.isOpenAccess | N | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordAuthor | Colitis | - |
dc.subject.keywordAuthor | Colon | - |
dc.subject.keywordAuthor | Epithelial Barrier | - |
dc.subject.keywordAuthor | IBD | - |
dc.subject.keywordAuthor | IPMK | - |
dc.subject.keywordAuthor | Single-Cell RNA-Seq | - |
dc.subject.keywordAuthor | Tuft Cell | - |
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