DC Field | Value | Language |
---|---|---|
dc.contributor.author | Lee, Sang Yup | ko |
dc.contributor.author | Jeong, Ki Jun | ko |
dc.date.accessioned | 2022-12-20T09:00:29Z | - |
dc.date.available | 2022-12-20T09:00:29Z | - |
dc.identifier.uri | http://hdl.handle.net/10203/303359 | - |
dc.description.abstract | The present invention provides a recombinant plasmid vector comprising a kanamycin resistance gene, a promoter, an endoxylanase signal sequence, a nucleotide sequence coding for an oligopeptide consisting of 13 amino acids including 6 consecutive histidine residues, and a human granulocyte colony stimulating factor (hG-CSF) gene; an E. coli transformed with the said vector; and, a process for producing complete hG-CSF protein with high purity from the protein pool secreted by the said microorganism. In accordance with the invention, the hG-CSF protein can be prepared with high purity through rather simple process facilitating secretion of large amount of hG-CSF fusion protein into the periplasm, which does not require complicated processes such as solubilization and subsequent refolding required for isolation of the hG-CSF protein produced in cytoplasm as insoluble inclusion bodies by conventional techniques, thus, the hG-CSF protein can be widely used as an active ingredient in the development of supplementary agents for anticancer therapy. | - |
dc.title | Escherichia coli Strain Secreting Human GranulocyteColony Stimulating Factor(G-CSF) | - |
dc.title.alternative | 인체 과립성 백혈구의 콜로니 자극인자를 분비하는 대장균 | - |
dc.type | Patent | - |
dc.type.rims | PAT | - |
dc.contributor.localauthor | Lee, Sang Yup | - |
dc.contributor.localauthor | Jeong, Ki Jun | - |
dc.contributor.assignee | KAIST | - |
dc.identifier.iprsType | 특허 | - |
dc.identifier.patentApplicationNumber | 01800753.8 | - |
dc.identifier.patentRegistrationNumber | 001156575 | - |
dc.date.application | 2001-03-31 | - |
dc.date.registration | 2004-07-07 | - |
dc.publisher.country | CC | - |
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