METHOD FOR DETECTING TARGET RNA BY UTILIZING NICKING/EXTENSION CHAIN REACTION SYSTEM- BASED ISOTHERMAL NUCLEIC ACID AMPLIFICATION핵산의 절단 및 중합연쇄반응 시스템 기반 등온 핵산증폭기술을 이용한 표적 RNA 검출 방법
The present invention relates to a detection method for detecting a target RNA contained in a sample with high sensitivity by using nicking/extension chain reaction system-based isothermal nucleic acid amplification (NESBA) that uses activity of a cleavage enzyme and a DNA polymerase. The NESBA of the present invention is a new concept isothermal target RNA detection method that realizes higher amplification efficiency than the existing NASBA technology and is deemed to be utilizable as a new concept diagnosis technology that can replace conventional target RNA detection technologies.